齿叶薰衣草中木犀草苷等4个成分的含量测定及指纹图谱研究

目的：建立齿叶薰衣草药材中木犀草苷、木犀草素-7-O-β-D-葡萄糖醛酸苷、迷迭香酸和木犀草素的含量测定方法，为其质量控制提供科学依据。方法：采用HPLC法测定；色谱条件：Phenomenex Gemini色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈和0.2%磷酸二元梯度洗脱，流速1.0 mL·min^-1，柱温30℃，检测波长350 nm。结果：木犀草苷、木犀草素-7-O-β-D-葡萄糖醛酸苷、迷迭香酸和木犀草素的进样量在0.004~0.20、0.008~0.39、0.002~1.05、0.004~0.22 μg范围内与峰面积呈良好的线性关系（r=0.999 8~0.999 9），平均回收率在101.3%%~103.6%之间（RSD < 2%，n=6）。建立11批齿叶薰衣草药材HPLC指纹图谱，获得11个共有峰，从中确定了4个化学成分。结论：该方法操作简便，重复性好，可用于规范和控制齿叶薰衣草药材的质量。

Objective: To establish the determination method luteoloside,luteolin-7-O-β-D-glucuronide,rosmarinic acid and luteolin in Lavandula dentata L.,so as to offer scientific basis for quality standard of the herb. Methods: The HPLC separation was performed on a Phenomenex Gemini column (250 mm×4.6 mm,5 μm) at 30with gradient elution of acetonitrile and 0.2% phosphate acid solution at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at 350 nm. Results: Luteoloside,luteolin-7-O-β-D-glucuronide,rosmarinic acid and luteolin exhibited good linearities (r=0.999 8-0.999 9) and the linear ranges were 0.004-0.20 μg,0.008-0.39 μg,0.002-1.05 μg and 0.004-0.22 μg,respectively. The average recoveries (RSD < 2%,n=6) were L.. between 101.3%-103.6%. HPLC fingerprints of 11 batches of Lavandula dentata L.11 common peaks were selected and 4 of them were identified. Conclusion: The method is simple and repeatable,and it can be used for the quality control of Lavandula dentata L..

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