Objective: To establish a specific, rapid and simple method for the determination of palmatine by quantitative nuclear magnetic resonance(qNMR). Methods: DMSO-d6 was employed as solvent, and maleic acid as an internal standard. The testing temperature was set at 300 K,pulse width was 9.54 μs,the delay time was 15 s and the number of scanning was 64. Under these conditions,the 1H-NMR data of palmatine was obtained,and then calculate the assay of palmatine by comparing the response signal area of palmatine(As) with that of maleic acid (Ar). Results: Proton signal peaks at δ7.10 of palmatine and δ6.26 of maleic acid served as quantitative peaks, which showed good separation. The precision RSD was 0.23%(n=6) and the repeatability RSD was 1.2%(n=6). Linear regression of quantitative peak areas ratio(As/Ar, X) of palmatine-maleic acid versus mass ratio(ms/mr, Y) yielded a correlation coefficient of 0. 999 9 and a regression equation of Y=3.782X+0.072. The contents of 3 batches of palmatine were 86.83%, 86.94% and 87.01%; the results were generally consistent with that of mass balance methods. Conclusion: This method is easy and simple to handle, and the analysis results are accurate. And the structures could also be identified at the same time. Therefore, qNMR could be used for the quality control of palmatine.
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