阳离子交换离子色谱法测定钙镁片中钙、镁离子含量

目的：建立采用离子色谱法测定保健食品钙镁片中钙离子和镁离子含量的方法。方法：样品经0.5mol·L^-1盐酸溶解后，经IonPac CS12A阳离子交换色谱柱分离，采用CSRS^® 300 4-mm阳离子抑制器，电导检测器检测，标准曲线法定量。结果：钙离子、镁离子质量浓度分别在1~20和0.5~10 mg·L^-1的范围内线性关系良好，相关系数皆大于0.999 7；检出浓度分别为0.002和0.001 mg·L^-1，定量浓度分别为0.006和0.003 mg·L^-1，回收率（n=6）分别为99.1%（RSD=1.9%）和99.4%（RSD=2.0%）； 5批样品钙离子的含量分别为13.6%、15.2%、14.9%、16.7%、10.8%，镁离子的含量分别为5.21%、3.86%、4.5%、6.01%、3.92%。结论：本法适用于钙镁片中钙、镁离子含量的测定。

Objective: To establish a method for the content determination of calcium ion and magnesium ion in health food calcium and magnesium tablets by ion chromatography. Methods: The samples were dissolved by 0.5 mol·L^-1 hydrochloric acid solution, and then separated by IonPac CS12A column. CSRS^® 300 4-mm cation inhibitor and conductivity detector were used. Standard curve method was used for quantitative analysis. Results: The calibration curves had good linearity within 1-20 mg·L^-1 for calcium ion and 0.5-10 mg·L^-1 for magnesium ion, r > 0.999 7. The limits of detection were 0.002 and 0.001 mg·L^-1 and the limits of quantitation were 0.006 and 0.003mg·L^-1 for calcium ion and magnesium ion, respectively. The recoveries(n=6) were 99.1% (RSD=1.9%)、99.4%(RSD=2.0%), respectively. The contents of calcium were 13.6%、15.2%、14.9%、16.7%、10.8% and the contents of magnesium were 5.21%, 3.86%, 4.5%, 6.01%, 3.92% in five samples. Conclusion: The established ion chromatographic method was simple, sensitive, rapidly, and can be used to analyze calcium ion and magnesium ion in the health food, calcium and magnesium tablets.

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