芸香浸膏的定性定量方法研究

目的：建立芸香浸膏的定性定量方法。方法：采用薄层色谱法，以正己烷-石油醚（60~90 ℃）-乙酸乙酯-冰醋酸（6：2：3：0.1）为展开剂，展开，置紫外光灯（254 nm）下检视，对枫香脂药材进行鉴别及对掺伪物松香进行检查；采用高效液相色谱法测定肉桂酸含量，使用Syncronis C₁₈（250 mm×4.6 mm，5 μm）色谱柱，以甲醇-1% 醋酸溶液（50：50）为流动相，流速1.0 mL·min^-1，检测波长为285 nm，柱温为30 ℃。结果：薄层色谱斑点清晰，分离度好；高效液相色谱法测定含量，肉桂酸质量浓度在1.66~41.52 μg·mL^-1（r=0.999 9）范围内呈良好线性关系，低、中、高浓度加样回收率分别为97.1%（RSD=1.7%）、101.0%（RSD=0.9%）和96.9%（RSD=1.1%）。在对12 个样品的检验中，含量在0.38%~14.09% 之间。结论：本文所建立的方法可以作为芸香浸膏的质量控制方法。

Objective: To establish the qualitative and quantitative methods of Yunxiang extraction. Methods: TLC identification method was used. Hexane-petroleum ether(60-90℃)-ethyl acetate-glacial acetic acid(6:2:3:0.1)was emplet employed as the developing agent,and the ultraviolet detection was carried out at 254 nm. TLC was applied to the identification of resina liquidambaries and the examination of rosin. HPLC was applied to the determination of cinnamic acid. The Syncronis C₁₈(250 mm×4.6 mm,5 μm)chromatographic column was used and the mobile phase consisted of methanol-1% acetic acid(50:50)at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at 285 nm and the column temperature was set at 30℃. Results: The spots of thin layer chromatography were clear with good resolution. The good linear range of cinnamic acid was 1.66-41.52 μg·mL^-1(r=0.999 9). The recovery of low,medium and high concentrations were 97.1%(RSD=1.7%),101.0%(RSD=0.9%)and 96.9% (RSD=1.1%). The content range of cinnamic acid in 12 samples was 0.38%-14.09%. Conclusions: The method can be used as the quality control method of Yunxiang extraction.

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