神香草抗哮喘有效部位定性定量研究

目的：建立神香草抗哮喘有效部位的定性定量控制方法。方法：采用高效液相色谱法测定神香草有效部位中的迷迭香酸，并建立特征图谱；采用紫外分光光度法测定神香草有效部位中的总黄酮和总多糖。结果：神香草抗哮喘有效部位中迷迭香酸含量为3.68%，总黄酮含量为35.10%，总多糖含量为17.12%，建立了以5个共有峰为指标成分的神香草有效部位HPLC特征图谱。结论：本研究所建立的定性定量控制方法，能全面评价神香草抗哮喘有效部位的质量，并可为研究新药奠定基础。

Objective:To establish a qualitative and quantitative control method for the effective parts of Hyssopus cuspidatus Boriss anti-asthmatic.Methods:An HPLC was used to measure the contents of rosmarinic acid in the effective parts of Hyssopus cuspidatus Boriss anti-asthmatic and the HPLC specific chromatogram was also established.UV was used to measure the contents of total flavonoids and total polysaccharide of the effective parts of Hyssopus cuspidatus Boriss anti-asthmatic.Results:The contents of rosmarinic acid,total flavonoids and total polysaccharide were 3.68%,35.10% and 17.12%,respectively;HPLC specific chromatogram consisted of five common peaks.Conclusion:The method established in this study can be applied in the quality control for qualitative study on and quantitative stady on the effective parts of Hyssopus cuspidatus Boriss anti-asthmatic and can lay a foundation for the study of new drugs.