蒙古族药塔日努及其不同炮制品HPLC指纹图谱研究

目的：建立蒙古族药材塔日努及其牛奶制、酒制及诃子汤制品指纹图谱，为塔日努不同炮制品质量控制及炮制原理研究提供依据。方法：采用HPLC，Hypersil C₁₈色谱柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.4%磷酸水，梯度洗脱，柱温40℃，检测波长254 nm，对塔日努及其3种炮制品进行指纹图谱研究，采用指纹图谱相似度软件进行分析。结果：建立了塔日努及其3种炮制品指纹图谱，塔日努经炮制后，其成分发生了变化，塔日努生品及牛奶、诃子汤制品的指纹图谱有明显区别，而生品塔日努及酒制塔日努指纹图谱较为相似。奶制品中5号锋消失，诃子汤制品中新增7号和8号锋、新增的7号峰峰面积较大。结论：该法精密度、重复性、稳定性良好，符合指纹图谱技术要求，可作为塔日努及其炮制品的质量控制方法。

Objective:To provide a basis for quality control and processing principle research of Tarinu and its different processed products by establishing HPLC fingerprints.Methods:HPLC was performed on a Hypersil C₁₈ column (4.6 mm×250 mm,5 μm)with mobile phase of acetonitrile-0. 4% phosphoric acid in gradient elution at a flow rate of 1.0 mL·min^-1.The column temperature was 40℃ and the detection wavelength was 254 nm. The fingerprints of Tarinu and its 3 kinds of processed products were analyzed by fingerprint similarity software.Results:There were significant differences in fingerprints of raw products and milk and Terminalia chebula Retz soup processing products. But fingerprints of raw products and alcohol processing products were relatively similar. Compared with the fingerprint of Tarinu,peak 7 and peak 8 were found as new components in Terminalia chebula Retz soup processing product and the peak area of peak 7 was relatively larger. On the other hand,peak 5 disappeared in milk processing product.Conclusion:The method is stable and accurate with good reproducibility and can be used in quality control of Tarinu and its processed products.

[1] 国家中医药管理局《中华本草》编委会.中华本草.蒙药卷[M].上海:上海科学技术出版社,2004:325"Chinese Materia Medica"editorial board of State Administration of Traditional Chinese Medicine.Chinese Materia Medica.Mongolian Medicine Volume[M].Shanghai:Shanghai Science and Technology Press,2004:325
[2] 哲里木盟蒙医研究所.医药月帝[M].赤峰:内蒙古科学技术出版社,2013:375 The Mongolian Medicine Research Institute of Jirem League.Medicine Moon Emperor[M].Chifeng:Inner Mongolia Science and Technology Press,2013:375
[3] 中华人民共和国药典2015年版.一部[S].2015:286 ChP 2015.VolⅠ[S].2015:286
[4] LI WJ,LIN Y,WANG YC,et al.Development of a matrix solid-phase dispersion extraction combined with UPLC/Q-TOF-MS for determination of phenolics and terpenoids from the Euphorbia fischeriana[J].Molecules,2017,22(9):1510
[5] WANG CJ,YAN QL,MA YF,et al.ent-Abietane and tigliane diterpenoids from the roots of Euphorbia fischeriana and their inhibitory effects against mycobacterium smegmatis[J].J Nat Prod,2017,80(5):1248
[6] CAI DF,ZHAI WY,ZHANG Q,et al.Development of matrix solid-phase dispersion coupled with high-performance liquid chromatography for determination of jolkinolide A and jolkinolide B in Euphorbia fischeriana Steud[J].Nat Prod Res,2017,31(15):1805
[7] LEE JW,LEE C,JIN QH,et al.Diterpenoids from the roots of Euphorbia fischeriana with inhibitory effects on nitric oxide production[J].J Nat Prod,2016,79(1):126
[8] SUN YX,LIU JC.Chemical constituents and biological activities of Euphorbia fischeriana Steud[J].Chem Biodiv,2011,8(7):1205
[9] XU L,ZHI GL,YUN FC,et al.Chemotaxonomic and chemical studies on two plants from genus of Euphorbia:Euphorbia fischeriana and Euphorbia ebracteolata[J].Biochem Syst Ecol,2014,57:345
[10] XIN ZK,WWI L,YUICHIRO K.Euphorins A-H:bioactive diterpenoids from Euphorbia fischeriana[J].J Nat Med,2016,70(3):412
[11] 王璞,王亚旭,王聿成,等.有毒蒙药常用减毒方法研究探讨[J].云南中医学院学报,2013,36(5):68 WANG P,WANG YX,WANG YC,et al.Study on common attenuation methods for toxic Mongolian drugs[J].J Yunnan Univ Tradit Chin Med,2013,36(5):68
[12] 丹增彭措.炮制明藏[M].赤峰:内蒙古科学技术出版社,2013:88 DANZENGPENGCUO.Processing the Ming Dynasty[M].Chifeng:Inner Mongolia Science and Technology Press,2013:88
[13] 王宏伟,王海香,顾雅静,等.狼毒大戟化学成分和药效作用研究进展[J].天然产物研究与开发,2012,24(12):1853 WANG HW,WANG HX,GU YJ,et al.The progress of chemical constituents of Euphorbia fischeriana and pharmacodynamic study[J].Nat Prod Res Dev,2012,24(12):1853
[14] 么焕开,张文婷,郑雪晶,等.狼毒大戟化学成分及药理作用研究进展[J].中成药,2010,32(8):1404 YAO HK,ZHANG WT,ZHENG XJ,et al.Research progress in chemical constituents and pharmacological effects of Euphorbia fischeriana Steud[J].Chin Tradit Pat Med,2010,32(8):1404