HPLC法同时测定龙胆泻肝丸中8个活性成分的含量

Objective: To develop an HPLC method for simultaneous determination of gentiopicroside,swertiamarin,geniposide,genipingentiobioside,baicalin,wogonoside,baicalein and wogonin in Longdan Xiegan pills.Methods: The chromatographic separation was performed on a SunFire^TM C₁₈ column(4.6 mm×250 mm,5 μm) with gradient elution(0-6 min,10%A→15%A;6-7 min,15%A;7-10 min,15%A→16%A;10-15 min,16%A;15-20 min,16%A→30%A;20-35 min,30%A→33%A;35-38 min,33%A→38%A;38-60 min,38%A→48%A) of acetonitrile(A) and 0.1% phosphoric acid(B) ataflow rate of 1.0 mL·min^-1.The detecting wavelength was set at 254 nm and the column temperature was maintained at 30℃.Results: The calibration curves were linear within the range of 2.5-100 μg·mL^-1(r=0.999 6) for gentiopicroside,2.5-100 μg·mL^-1(r=0.999 6) for swertiamarin,5-200 μg·mL^-1(r=0.999 6) for geniposide,2.5-100 μg·mL^-1(r=0.999 7) for genipingentiobioside,5-200 μg·mL^-1(r=0.999 5) for baicalin,2.5-100 μg·mL^-1(r=0.999 6) for wogonoside,1.25-50 μg·mL^-1(r=0.999 8) for baicalein and 1.25-50 μg·mL^-1(r=0.999 8) for wogonin.The recoveries(n=9) of gentiopicroside,swertiamarin,geniposide,genipingentiobioside,baicalin,wogonoside,baicalein and wogonin were 99.4%,99.2%,99.3%,98.7%,99.6%,98.7%,98.2%and 98.0%,respectively.The content ranges of eight components in six batches were 1.700-1.717 mg·g^-1 for gentiopicroside,0.524-0.544mg·g^-1 for swertiamarin,3.674-3.883 mg·g^-1 for geniposide,1.695-1.701 mg·g^-1 for genipingentiobioside,7.235-7.440 mg·g^-1 for baicalin,1.233-1.275 mg·g^-1 for wogonoside,0.514-0.523 mg·g^-1 for baicalein and 0.294-0.298 mg·g^-1 for wogonin.Conclusion: The method can be used for the quality control of Longdan Xiegan pills.