太子参萃取物抗应激损伤所致炎症反应机制研究

目的：研究3种不同萃取方式对太子参萃取物的抗炎症反应功效的差异，为太子参在抗炎症方面的应用提供参考。方法：分别采用水加热回流萃取，95%乙醇超声波萃取和超临界萃取法萃取太子参获得不同萃取物，利用MTT实验评估萃取物的安全性，脂氧合酶活性抑制试验和一氧化氮清除实验评估太子参萃取物的抗炎活性，运用pUC119 DNA质粒模型评估太子参萃取物的DNA保护作用。结果：太子参水萃取物和醇萃取物具有显著抑制脂氧合酶活性，活性由强至弱的顺序为水萃取物、醇萃取物；3种方式所得太子参萃取物均具有清除NO活性，活性由强至弱的顺序为水萃取物、超临界萃取物、醇萃取物；太子参水萃取物和醇萃取物均具有一定的DNA保护作用。结论：太子参萃取物抗应激损伤与其具有抑制脂氧合酶活性，清除NO和DNA保护功能有关。

Objective: To study anti-inflammation effect of Pseudostellaria heterophylla extracts by three different extraction methods, providing information for the application to anti-inflammation of Pseudostellaria heterophylla. Methods: Water heating reflux extraction, 95% ethanol ultrasonic extraction and super critical extraction were used for extraction, separately. MTT method was used to assess the toxicity. Lipoxygenase(LOX-1)activity inhibition test and nitric oxide scavenging experiment were used for the analysis of anti-inflammatory activity. The pUC119 DNA plasmid model was applied to examine the DNA protection effect. Results: Water extracts and alcohol extracts of Pseudostellaria heterophylla significantly inhibited lipoxygenase activity, and the intensity of aqueous extracts and alcohol extracts were in an descending order. Extracts from Pseudostellaria heterophylla in three ways all showed NO-elimination activity, and the intensity of aqueous extracts, supercritical extracts, and alcohol extracts were in descending order. Water extracts and alcohol extracts both had certain protective effect for DNA. Conclusion: The ability of Pseudostellaria heterophylla extracts in helping stressin juries is related to its lipoxygenase activity inhibition, NO-scavenging, and DNA protection activity.