川芎多波长叠加指纹图谱初步研究

目的：采用反相高效液相色谱法对川芎多波长叠加指纹图谱进行研究。方法：采用Poroshell120 EC-C₁₈色谱柱（4.6 mm×100 mm，2.7μm），以0.1%磷酸水溶液（A）-乙腈（B）为流动相梯度洗脱（0~20 min，10% → 32%的B；20~25 min，32% → 46%的B；25~36 min，46% → 55%的B；36~60 min，55% → 80%的B；60~63 min，80% → 10%的B；63~70 min，10%的B），流速0.8 mL·min^-1，柱温35℃，检测波长230、260、290、320 nm。运用波长叠加技术建立指纹图谱。结果：多波长叠加指纹图谱体现了川芎230、260、290、320 nm特征吸收波长的指纹信息，标定了22个共有峰，16个不同来源的川芎样品与指纹图谱之间具有良好的相似性，相似度均在0.94以上。结论：建立了川芎多波长叠加谱指纹图谱，该指纹图谱信息更加丰富全面，可用于川芎的质量控制。

Objective: To study and establish the multi-wavelength overlapping HPLC fingerprint of Chuanxiong Rhizoma. Methods: The separation was performed on an Agilent Poroshell 120 EC-C₁₈ column(4. 6 mm×100 mm, 2. 7μm)and the mobile phase was composed of 0. 1% phosphoric acid(A)-acetonitrile(B)with gradient elution(0-20 min, 10% → 32% B; 20-25 min, 32% → 46%B; 25-36 min, 46% → 55%B; 36-60 min, 55% → 80%B; 60-63 min, 80% → 10%B; 63-70 min, 10%B)at a flow rate of 0. 8 mL·min^-1. The column temperature was maintained at 35℃ and the detection wavelength was set at 230, 260, 290 and 320 nm. The fingerprint was established by using of multi-wavelength overlapping technique. Results: The multiwavelength overlapping HPLC fingerprint revealed information at 230, 260, 290 and 320 nm, and 22 common peaks were identified. 16 samples from different sources were with similarities all above 0. 94. Conclusion: The multi-wavelength overlapping fingerprint of Chuanxiong Rhizoma was established boasting abundant information, which could be used in quality control of Chuanxiong Rhizoma.