目的：建立HPLC波长切换法测定复方黄芩片中虎杖苷、黄芩苷、汉黄芩苷、黄芩素、穿心莲内酯、脱水穿心莲内酯的含量。方法：采用Agilent SB-C18（4.6 mm×150 mm，5 μm）色谱柱，流动相为乙腈（A）-0.1磷酸水溶液（B）系统，梯度洗脱，流速为1.0 mL·min-1，柱温：30℃，波长切换时间序列采样（0~10 min，306 nm，检测虎杖苷；10~23 min，280 nm，检测黄芩苷；23~25min，225 nm，检测穿心莲内酯；25~33 min，280 nm，检测汉黄芩苷、黄芩素；33~50 min，254 nm，检测脱水穿心莲内酯）。结果：虎杖苷进样量在0.046~0.823 μg范围内线性关系良好（r=0.999 9，n=6），平均回收率为99.30%，RSD为1.5%（n=6）；黄芩苷进样量在0.468~8.428 μg范围内线性关系良好（r=0.999 7，n=6），平均回收率为99.76%，RSD为1.3%（n=6）；汉黄芩苷进样量在0.116~2.084 μg范围内线性关系良好（r=0.999 9，n=6），平均回收率为100.3%，RSD为1.8%（n=6）；黄芩素进样量在0.117~2.110 μg范围内线性关系良好（r=0.999 8，n=6），平均回收率为99.25%，RSD为1.3%（n=6）；穿心莲内酯进样量在0.029~0.524 μg范围内线性关系良好（r=0.999 8，n=6），平均回收率为99.91%，RSD为1.6%（n=6）；脱水穿心莲内酯进样量在0.033~0.592 μg范围内线性关系良好（r=0.999 7，n=6），平均回收率为99.94%，RSD为1.6%（n=6）。5批样品中虎杖苷、黄芩苷、汉黄芩苷、黄芩素、穿心莲内酯和脱水穿心莲内酯含量范围分别为2.09~2.25、22.74~25.91、5.69~6.08、5.74~6.11、1.27~1.41、1.49~1.69 mg·g-1。结论：该方法经方法学验证，可用于复方黄芩片的质量控制。
Objective: To establish an HPLC method for simultaneous determination of polydatin, baicalin, wogonoside, baicalein, andrographolide, and dehydroandrographolide in Fufang Huangqin tablets. Methods: The separation was performed on an Agilent SB-C18 column(4.6 mm×150 mm, 5 μm) with gradient elution at a temperature of 30℃. The acetonitrile and 0.1%(v/v) phosphoric acid were used as the mobile phase with a flow rate of 1.0 mL·min-1. The detection wavelength was 306 nm(0-10 min) for polydatin,280 nm(10-23 min) for baicalin, 225 nm(23-25 min) for andrographolide, 280 nm(25-33 min) for wogonoside and baicalein,and 254 nm(33-50 min) for dehydroandrographolide. Results: Polydatin had a good linearity in the range of 0.046-0.823 μg,the average recovery was 99.30% with RSD of 1.5%(n=6);baicalin had a good linearity in the range of 0.468-8.428 μg, the average recovery was 99.76% with RSD of 1.3%(n=6);wogonoside had a good linearity in the range of 0.116-2.084 μg,the average recovery was 100.3% with RSD of 1.8%(n=6);baicalein had a good linearity in the range of 0.117-2.110 μg,the average recovery was 99.25% with RSD of 1.3%(n=6);andrographolide had a good linearity in the range of 0.029-0.524 μg,the average recovery was 99.91% with RSD of 1.6%(n=6);dehydroandrographolide had a good linearity in the range of 0.033-0.592 μg,the average recovery was 99.94% with RSD of 1.6%(n=6).The contents of polydatin,baicalin,wogonoside,baicalein,andrographolide and dehydroandrographolide in 5 samples were 2.09-2.25, 22.74-25.91,5.69-6.08,5.74-6.11,1.27-1.41 and 1.49-1.69 mg·g-1,respectively. Conclusion: The established method is validated by methodology that it is suitable for the quality control of Fufang Huangqin tablets.
