桂皮醛在常用溶剂中稳定性考察—对中国药典2015年版桂皮醛含量测定方法的探讨

目的：观察桂皮醛在常用有机溶剂中的稳定性，对中国药典2015年版（一部）中收载的桂皮醛含量测定方法进行探讨。方法：将桂皮醛对照品分别溶于甲醇、无水乙醇、乙腈、丙酮、乙酸乙酯等溶剂，采用HPLC和GC法测定，测定其色谱纯度的初始值及随时间变化情况。结果：桂皮醛在甲醇、无水乙醇、丙酮中不稳定，在乙腈、乙酸乙酯中稳定。结论：验证了桂皮醛GC含量测定方法采用乙酸乙酯制备对照品溶液的合理性，同时建议桂皮醛HPLC含量测定方法中改用乙腈制备对照品溶液。

Objective: To investigate stability of cinnamaldehyde in common organic solvents,and discuss the assay of cinnamaldehyde in Chinese pharmacopoeia (ChP),2015 edition,VolⅠ.Methods: The cinnamaldehyde chemical reference substance was dissolved in methanol,anhydrous ethanol,acetonitrile,acetone,acetonitrile and ethyl acetate,respectively.Then,HPLC and GC was used to determine the solvents at different time period.The initial value and changing trend of the chromatographic purity of cinnamaldehyde was calculated. Results: The results indicated that cinnamaldehyde was not stable in methanol,anhydrous ethanol or acetone,while stable in acetonitrile and ethyl acetate.Conclusion: The investigation confirmed the rationality of the assay method of cinnamaldehyde by GC method,using ethyl acetate to prepare the reference solution.It is suggested that the solvent for reference solution in assay of cinnamaldehyde by HPLC method should be changed to acetonitrile.

[1] 张荣发.桂皮醛的药理作用研究进展[J].中国药业,2008,17(10):75 ZHANG RF.Research progress of pharmacological action of cinnamaldehyde[J].China Pharm,2008,17(10):75
[2] 方娆莹,张电光,陈锡炎,等.气相色谱法同时测定心宝丸中冰片、桂皮醛、麝香酮的含量[J].今日药学,2015,25(11):85 FANG XY,ZHANG DG,CHEN XY,et al.GC Determination of synthetic borneol,cinnamaldehyde and muscone in Xinbao pills[J].Pharm Today,2015,25(11):85
[3] 李莉,冯孟鑫,马恺悦,等.高效液相二极管阵列检测法比较巴西肉桂CANELA与中国肉桂中桂皮醛的含量[J].环球中医药,2015,8(11):1284 LI L,FENG MX,MA KY,et al.Comparison of the contents of cinnamaldehyde between Brazilian Cinnamomum zeylanicum Bl.and Chinese Cinnamomum zeylanicum Bl.by HPLC-DAD[J].Glob Tradit Chin Med,2015,8(11):1284
[4] 刘丹萍,赵惠玲,刘海燕,等.HPLC法同时测定桂枝配方颗粒中肉桂酸和桂皮醛的含量[J].亚太传统医药,2015,11(18):11 LIU DP,ZHAO HL,LIU HY,et al.Determine Guizhi formula grains cinnamic acid and cinnamic aldehyde content by HPLC[J].Asia-Pac Tradit Med,2015,11(18):11
[5] 徐洋洋,王添敏,初正云,等.HPLC-DAD测定5种商品规格肉桂及两种伪品中桂皮醛的含量[J].中国实验方剂学杂志,2014,20(1):90 XU YY,WANG TM,CHU ZY,et al.Determination of cinimaldehyde in 5 different standards of Cinnamomum cassia and two kinds of adulterants by HPLC-DAD[J].Chin J Exp Tradit Med Form,2014,20(1):90
[6] 丁菊英,王晓娜,王晓坤.HPLC测定小儿温里止泻贴剂中桂皮醛的含量[J].食品与药品,2014,16(3):210 DING JY,WANG XN,WANG XK.Determination of cinnamaldehyde in Xiaoer Wenlizhixie patch by HPLC[J].Food Drug,2014,16(3):210
[7] 刘宏明,张叶,聂磊,等.HPLC法同时测定小儿柴桂退热颗粒中葛根素、大豆苷、黄芩苷、大豆苷元、桂皮醛、黄芩素的含量[J].药物分析杂志,2014,34(6):1038 LIU HM,ZHANG Y,NIE L,et al.Simultaneous determination of puerarin,daidzin,baicalin,daidzein,cinnamaldehyde and baicalein in Xiaoer Chaigui Tuire Keli by HPLC[J].Chin J Pharm Anal,2014,34(6):1038
[8] 中国药典2015年版.一部[S].2015 ChP 2015.VolⅠ[S].2015