2种试剂对实验动物凝血酶原时间检测结果的比对分析

目的：比对分析仪器配套的2种试剂对不同实验动物凝血酶原时间测定值，评价检测结果是否存在差异。方法：用Thromborel-S（TS）与Thromboplastin.C.Plus（CPLUS）2种凝血酶原试剂分别对实验动物标本进行检测，然后对检测结果进行配对t检验、相关性分析及信度检验。结果：不同实验动物凝血酶原时间，CPLUS试剂检测结果高于TS试剂，且两组数据存在显著性差异。结论：TS试剂与CPLUS试剂对实验动物凝血酶原时间的检测结果存在显著差异，不存在一致性，在同一个毒性研究中不可以交叉使用这2种试剂。

Objective: To compare and analyze the prothrombin time between two reagents matched with instrument on laboratory animals,and to explore the differences between these two reagents.Methods: Specimens from laboratory animals were tested using Thromborel-S(TS)and Thromboplastin. C. Plus(CPLUS)reagents,data were analyzed by paired t test,simple correlation analysis and intra-class correlation analysis.Results: Compared to TS,prothrombin time result was higher when a specimen was tested with CPLUS.There were statistically significant differences between the two groups of data obtained from two reagents separately.Conclusions: Different reagents can result in statistically significant differences in the prothrombin time with poor consistency,so these two kinds of reagents cannot be used cross on individual toxicity study.

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