参芪归芍汤化学成分的UPLC-Q-TOF/MS分析

目的：采用超高效液相色谱-飞行时间质谱（UPLC-Q-TOF/MS）对参芪归芍汤中的化学成分进行分析和鉴定。方法：采用ACQUITYTM UPLC BEH C₁₈（100 mm×2.1 mm，1.7 μm）色谱柱，以0.1%甲酸-乙腈为流动相进行快速梯度洗脱，流速1.0 mL·min^-1，柱温35 ℃，进样量10 μL。电喷雾离子源负离子模式，毛细管电压分别为1.4 kV和1.3 kV，锥孔电压分别为40 V和23 V，离子源温度120 ℃，脱溶剂气温度350 ℃，锥孔气流量50 L·h^-1，脱溶剂气流量600 L·h^-1，碰撞能量10~40 eV，离子能量1 eV。结果：从参芪归芍汤中鉴定了42个化合物，结果显示，参芪归芍汤中的主要化学成分包括皂苷、黄酮和酚酸等类化合物。结论：该研究比较全面地阐明了参芪归芍汤的化学组成，对参芪归芍汤的质量控制和药效物质基础的研究具有十分重要意义。

Objective: To analyze and identify the chemical constituents of Shenqi Guishao decoction by ultra performance liquid chromatography time-of-flight mass spectrometry(UPLC-Q-TOF/MS). Methods: The separation was performed on an ACQUITYTM UPLC BEH C₁₈ Column(100 mm×2.1 mm,1.7 μm)with gradient elution,which was carried out with 0.1% formic acid acetonitrile as mobile phase. The flow rate was 1.0 mL·min^-1,the temperature of column was 35 ℃,and the injection volume was 10 μL. Electrospray ionization source was in negative ion mode,capillary voltage was 1.4 kV and 1.3 kV,cone voltage was 40 V and 23 V,ion source temperature was 120 ℃,removal of solvent gas temperature was 350 ℃,cone gas flow was 50 L·h^-1,de-solvation gas flow was 600 L·h^-1,collision energy was 10 to 40 eV,and the ion energy was 1 eV. Results: Forty-two peaks were identified on line using the established method. The results implied that saponins,flavonoids and phenolic acids were main components of Shenqi Guishao decoction. Conclusion: The study has comprehensively illustrated the chemical composition of Shenqi Guishao decoction,which is significant to the quality control and the study of material basis of efficacy of Shenqi Guishao decoction.