甲酸结合不同真菌诱导形成的人工沉香质量评价研究

目的：建立气相色谱-质谱（GC-MS）联用技术的沉香指纹图谱，结合多元统计分析评价甲酸结合不同真菌诱导白木香生产人工沉香品质。方法：采用GC-MS联用技术建立40批沉香指纹图谱，使用《中药指纹图谱相似度评价（2004 A版）》软件建立共有模式，计算图谱相似度，标定共有峰。峰面积归一化法计算170~270 min总峰面积与0~100 min总峰面积两区间的比值。正交偏最小二乘法（OPLS-DA）对样品进行分组，变量权重（VIP）结合相关系数（P（corr））筛选差异性组分。自动质谱退卷积定性系统（AMDIS）结合保留指数（RI）对共有组分和差异组分进行鉴定。结果：10批天然沉香指纹图谱相似度为0.821~0.888，30批人工沉香与天然沉香生成的对照图谱相比相似度在0.307~0.960之间。40批沉香按峰面积比值0.65分为3组：天然沉香、人工沉香1和人工沉香2，且全谱碎片信息、共有组分和差异组分相对峰面积的OPLS-DA分析结果一致。10个共有组分和14个差异组分中，白木香醛等对天然沉香的分组呈正相关；愈创木醇等对人工沉香1分组呈正相关；角鲨烯、5，8-二羟基-2-（2-苯乙基）色酮等对人工沉香2分组呈负相关。结论：指纹图谱相似度、两区间总峰面积比值结合多元统计分析结果，可以快速判断人工诱导沉香品质，为沉香药材的全面质量评价提供参考依据。

Objective: To establish fingerprints of agarwood by gas chromatography-mass spectrometry(GC-MS),so as to evaluate the quality of artificial agarwood induced by formic acid stimulation combined with different fungal inoculation.Methods: The fingerprints of forty batches of agarwood were established by GC-MS.Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(Version 2004 A)was employed to calculate the similarity of each chromatogram of agarwood.The ratios of sum peak area in the range of 170-270 min of individual chromatogram and that in the range of 0-100 min were calculated by square peaks for normalization.Agarwood samples are divided by the result of OPLS-DA,using VIP combined with P(corr)to screen out the different components.AMDIS and RI were employed to identify the common and different components.Results: The correlation coefficient of natural agarwoods ranged from 0.821-0.888,while the correlation coefficient of artificial agarwood was 0.307-0.960.The OPLS-DA results showed that 40 batches of agarwood were separated into three groups according to the proportion of peak area(>0.65 or <0.65),which exhibited good consistency.Meanwhile,the results of mass ions of 40 batches of agarwood and the relative peak area of common and different components are the same.Among the 10 common components and 14 different components,baimuxinal for natural agarwood group was positively correlated,guaiol has positive correlation to artificial agarwood Ⅰ group,and Squalene and 5,8-dihydroxy-2-(2-phenylethyl)chromone for artificial agarwood Ⅱ group was negatively correlated.Conclusions: It is feasible to evaluate the quality of artificial agarwood via the combination of three aspects, i.e. similarity of fingerprint chromatographic, peak area ratio of two intervals and multivariate statistical analysis method, which also provides a reference for the comprehensive quality evaluation of agarwood.