HPLC法同时测定委陵菜中5个黄酮类成分的含量

目的：建立HPLC 法同时测定委陵菜中芦丁、金丝桃苷、槲皮素、芹菜素和山柰酚5 个成分含量。方法：采用ZORBAX SB-C18（4.6 mm×250 mm，5 μm）色谱柱，以乙腈-0.2% 磷酸水溶液（5:95）为流动相A，乙腈-0.2% 磷酸水溶液（70:30）为流动相B，梯度洗脱，流速为1 mL·min^-1，检测波长为320 nm。结果：在上述条件下，芦丁、金丝桃苷、槲皮素、芹菜素和山柰酚的质量浓度分别在12.50~200、6.25~100、6.25~100、6.25~100 和4.00~64.00 μg·mL^-1 范围内与峰面积呈良好的线性关系，平均加样回收率（n=9）均高于95%，RSD 小于3.0%，10 批样品中芦丁、金丝桃苷、槲皮素、芹菜素和山柰酚含量范围分别为144.7~342.4、117.9~232.7、123.3~203.6、65.7~142.6、43.3~89.3 μg·g^-1。结论：方法学验证结果表明，本法适用于委陵菜中黄酮类成分的含量测定。

Objective:To establish a method for simultaneous determination of rutin,hyperin,quercetin,apigenin and kaempferol in Potentilla chinensis Ser.by HPLC.Methods:HPLC separation was carried on a ZORBAX SBC 18 column(4.6 mm×250 mm,5 μm)with the mobile phase consisted of mobile phase A［acetonitrile-0.2% phosphoric acid solution system(5:95)］ and mobile phase B ［acetonitrile-0.2% phosphoric acid solution system (70:30)］ in a programme of gradient elution.The flow rate was 1 mL·min^-1 and the detection wavelength was set at 320 nm.Results:Under the above conditions,the linear ranges of rutin,hyperin,quercetin,apigenin and kaempferol were 12.50-200 μg·mL^-1,6.25-100 μg·mL^-1,6.25-100 μg·mL^-1,6.25-100 μg·mL^-1,and 4.00-64.00 μg·mL^-1; the average recoveries(n=9)for the sample preparation of the markers were more than 95.0% and RSDs were less than 3.0%;the content ranges of rutin,hyperin,quercetin,apigenin and kaempferol in 10 batches of samples were 144.7-342.4,117.9-232.7,123.3-203.6,65.7-142.6 and 43.3-89.3 μg·g^-1,respectively.Conclusion:The established method is proved by methodology validation to be suitable for content determination of flavonoids in Potentilla chinensis Ser.

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