银杏叶提取物中总银杏酸HPLC法限量检测
Limit determination of total ginkgolic acid in Ginkgo biloba extract by HPLC
分类号：
出版年·卷·期（页码）：2015,35 (11):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的:建立银杏叶提取物中总银杏酸HPLC限量检测方法。方法:银杏叶提取物以甲醇直接溶解,制备供试品溶液;HPLC条件:以十八烷基硅烷键合硅胶为填充剂,0.1%三氟乙酸乙腈为流动相A,0.1%三氟乙酸水为流动相B,梯度洗脱(0~30 min,75%A→90%A;30~35 min,90%A),流速1 mL·min-1,检测波长310 nm。结果:白果新酸的线性关系良好,平均回收率(n=9)为93.8%。6个厂家10批银杏叶提取物依法进行测定,结果显示:不同厂家提取物总银杏酸含量存在较大差异。结论:该法经方法学验证,可用于银杏叶提取物中总银杏酸的限量检测。
-----英文摘要：---------------------------------------------------------------------------------------
Objective:To establish a method for the limit determination of total ginkgolic acid in Ginkgo biloba extract by HPLC.Methods:Ginkgo biloba extract was dissolved with methanol.The determination was performed on C18 column with 0.1% trifluoroacetic acid in acetonitrile as the mobile phase A and 0.1% trifluoroacetic acid in water as the mobile phase B with gradient elution mode(0-30 min,75%A→90%A; 30-35 min,90%A),at a flow rate of 1 mL·min-1.The detection wavelength was 310 nm.Results:There was a good linear relationship with ginkgoneolic acid,and the average recovery(n=9)was 93.8%.Ten batches of Ginkgo biloba extract from six manufacturers were determined.Extracts from different manufacturers showed significant differenes of content of total ginkgo acid.Conclusion: Through the validation of mothodology,this method can be used as the limit determination of ginkgolic acid.
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