海勒氏液中苯甲酸、水杨酸的含量测定

目的:建立HPLC法测定海勒氏液中苯甲酸、水杨酸含量,提高海勒氏液的质量标准。方法:采用十八烷基硅烷键合硅胶色谱柱(150 mm×4.6 mm,5μm)色谱柱,以甲醇-0.1%磷酸(50:50)为流动相,流速1.0 mL·min^-1;检测波长240 nm;柱温35℃。结果:苯甲酸及水杨酸线性范围分别为7.95~119.25 μg·mL^-1,(r=1.0000)和5.06~75.97 μg·mL^-1(r=0.9999);平均回收率(n=9)分别为99.6%、99.3%、98.8%和98.2%、99.2%、99.7%。3批样品中苯甲酸含量分别为91.5、76.6和89.9 mg·mL^-1,水杨酸含量分别为50.4、48.4和49.9 mg·mL^-1。结论:该方法可用于海勒氏液中苯甲酸和水杨酸的含量测定。

Objective:To establish an HPLC method for determination of benzoic acid and salicylic acid,and improve the quality standard of Haileshi Ye.Methods:HPLC analysis was carried out with VP-ODS column(150 mm×4.6 mm,5 μm)and methanol-0.1% phosphoric acid solution(50:50)at a flow rate of 1.0 mL·min^-1.The detection wavelength was at 240 nm,and the column temperature was 35℃. Results:The linear ranges of benzoic acid and salicylic acid were 7.95-119.25 μg·mL^-1(r=1.000 0)and 5.06-75.97 μg·mL^-1(r=0.999 9),respectively;and the average recoveries(n=9)were 99.6%,99.3%,98.8% and 98.2%,99.2%,99.7%,respectively.The contents of benzoic acid and salicylic acid in 3 samples were 91.5,76.6, 89.9 mg·mL^-1 and 50.4,48.4,49.9 mg·mL^-1,respectively.Conclusion:This method can be used for the determination of benzoic acid and salicylic acid in Haileshi Ye.

[1] FU F(傅峰),SHI XJ(施孝金),GUO YP(郭燕萍),et al.Simultaneous content determination of benzoic acid and salicylic acid in a new concentrated benzoic acid and salicylic acid ointment by RP-HPLC(RP-HPLC法同时测定浓苯甲酸水杨酸软膏中苯甲酸和水杨酸的含量)[J].Chin J Clin Pharm(中国临床药学杂志),2012,21(3):160
[2] XING JJ(邢俊家),ZHOU H(周晖).Determination of benzoic acid and salicylic acid in compound benzoic acid gel by high-performance liquid-affinity chromatography(高效液相色谱法测定复方苯甲酸凝胶中苯甲酸和水杨酸的含量)[J].J China Med Univ(中国医科大学学报),2007,36(2):223
[3] LI HY(李焕宇),DENG ZH(邓朝晖).Determination of benzoic acid and salicylic acid in compound benzoic acid with iodine alcoholic solution by RP-HPLC(RP-HPLC法测定含碘复方苯甲酸醇溶液中苯甲酸和水杨酸的含量)[J].Pharm J Chin PLA(解放军药学学报),2013,29(3):247
[4] BAI L(白林),ZHU XJ(朱小洁).Determination of benzoic acid and salicylic acid in four compound preparations by HPLC(HPLC法同时测定四种复方制剂中苯甲酸和水杨酸的含量)[J].Pharm J Chin PLA(解放军药学学报),2014,30(1):66
[5] LAI WH(赖文红),HUANG L(黄力),SU F(苏芳).Study on quality standard of compound benzoic acid with iodine alcoholic solution(含碘复方苯甲酸醇溶液的质量标准研究)[J].Pharm J Chin PLA(解放军药学学报),2013,29(1):60
[6] GUO XG(郭旭光).Simultaneous determination of benzoic acid and salicy acid in salicy acid complex lotion by HPLC(HPLC法同时测定水杨酸复合洗剂中苯甲酸和水杨酸的含量)[J].China Pharm(中国药房),2014,25(28):2667
[7] NIAN SH(年四辉),LI P(李萍),LIU LM(刘丽敏),et al.Determination of salicylic acid and benzoic acid in Radix Isatidis and its preparations by HPLC(HPLC测定板蓝根及其制剂中水杨酸、苯甲酸的含量)[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2012,18(21):92
[8] LI Y(李莹),DAI B(戴博),SONG Y(宋妍).Study on the quality standard of tri-acid powder(三酸散的质量标准提高)[J].Chin Pharm Aff(中国药事),2014,28(9):1005
[9] HUANG L(黄力),GAO C(高从),LAI WH(赖文红),et al.Determination of benzoic acid and salicylic acid in tri-acid powder by HPLC(HPLC法测定三酸散中苯甲酸和水杨酸的含量)[J].Pharm J Chin PLA(解放军药学学报),2014,30(1):58
[10] YU XP(余小平),SHU JF(舒金富),HUANG H(黄华),et al.Content determination of salicylic acid and benzoic acid in huatuo ointment by HPLC(HPLC法同时测定华佗膏中水杨酸苯甲酸的含量)[J].Drug Stand China(中国药品标准),2010,11(5):375
[11] YU XP(余小平).HPLC Determination of content of salicylic acid and benzoic acid in Zuguansan(高效液相色谱法同时测定足光散中水杨酸、苯甲酸的含量)[J].Chin J Pharm Anal(药物分析杂志),2005,25(8):955
[12] HUI YH(惠毅红),LI N(李宁).Determination of content of benzoic acid and salicylic acid in the compound benzoic acid ointment by HPLC(高效液相色谱法测定复方苯甲酸软膏中苯甲酸及水杨酸的含量)[J].Mod Instrum(现代仪器),2009(6):72