RP-HPLC法测定人参花中11个人参皂苷含量

目的: 测定鲜人参花和人参花干品中11个人参皂苷的含量。方法: 利用反相高效液相色谱法,使用Cosmosil 5 C₁₈-MS(250 mm×4.6 mm,5 μm)分析柱,流动相为乙腈-0.05 mol·L^-1磷酸二氢钾溶液,梯度洗脱,流速1 mL·min^-1,柱温25℃,检测波长203 nm。结果: 丙二酰基人参皂苷Rb₁、Rb₂、Rc、Rd及人参皂苷Rb₁、Rc、Rb₂、Rd、Re、Rg₁、Rg₂质量浓度分别在18.83~301.20 μg·mL^-1(r=0.999 1)、19.01~304.20 μg·mL^-1(r=0.999 8)、18.94~303.00 μg·mL^-1(r=0.999 3)、18.79~300.60 μg·mL^-1(r=0.999 4)、18.56~297.00 μg·mL^-1(r=0.999 0)、18.86~301.80 μg·mL^-1(r=0.999 1)、18.94~303.00 μg·mL^-1(r=0.999 5)、37.58~601.20 μg·mL^-1(r=0.999 6)、37.58~601.20 μg·mL^-1(r=0.999 1)、19.05~304.80 μg·mL^-1(r=0.999 3)、18.68~298.80 μg·mL^-1(r=0.999 6)范围内与峰面积呈良好的线性关系,平均加样回收率(n=6)在98.3%~102.9%之间,RSD≤3.0%。鲜人参花中11个人参皂苷平均含量为10.80%,其中丙二酰基人参皂苷的平均含量为3.46%;人参花干品中11个人参皂苷含量为10.68%,其中丙二酰基人参皂苷的平均含量为1.21%。结论: 本实验方法简单,可高效地测定鲜人参花和人参花干品中11个人参皂苷的含量。

Objective: To establish a method for the determination of 11 kinds of ginsenosides from both fresh and dried Ginseng Bud. Methods: RP-HPLC was conducted on a Cosmosil 5 C₁₈-MS column(250 mm×4.6 mm,5 μm)with the mobile phase of acetonitrile-0.05 mol·L^-1 potassium dihydrogen phosphate solution by gradient elution at a flow rate of 1 mL·min^-1.The column temperature was maintained at 25℃,and the detection wave length was 203 nm. Results: The linear response ranged from 18.83-301.20 μg·mL^-1 for malonyl ginsenoside Rb₁(r=0.999 1),19.01-304.20 μg·mL^-1 for malonyl ginsenoside Rb₂(r=0.999 8),18.94-303.00 μg·mL^-1 for malonyl ginsenoside Rc(r=0.999 3),18.79-300.60 μg·mL^-1 for malonyl ginsenoside Rd(r=0.999 4),18.56-297.00 μg·mL^-1 for ginsenoside Rb₁(r=0.999 0),18.86-301.80 μg·mL^-1 for ginsenoside Rc(r=0.999 1),18.94-303.00 μg·mL^-1 for ginsenoside Rb₂(r=0.999 5),37.58-601.20 μg·mL^-1 for ginsenoside Rd(r=0.999 6),37.58-601.20 μg·mL^-1 for ginsenoside Re(r=0.999 1),19.05-304.80 μg·mL^-1 for ginsenoside Rg₁(r=0.999 3)and 18.68-298.80 μg·mL^-1 for ginsenoside Rg₂(r=0.999 6).The average recoveries(n=6)were between 98.3%-102.9%,and the RSD ≤3.0%.In fresh Ginseng Bud,the contents of 11 kinds of ginsenosides was 10.80%,and the content of malonyl ginsenosides was 3.46%;in dried Ginseng Bud,the contents of 11 kinds of ginsenosides was 10.68%,and the content of the malonyl ginsenosides was 1.21%. Conclusion: The established method is simple,fast and accurate,and can be applied for the determination of the content of 11 kinds of ginsenosides from both fresh and dried Ginseng Bud.