杜仲不同炮制品降压活性的比较研究

目的: 研究比较杜仲不同炮制品的降压活性。方法: 选用雄性自发性高血压大鼠(spontaneously hypertension rat stroke-prone,SHR-SP),按血压值高低进行随机区间分组,包括杜仲生品组、杜仲炮制品组、杜仲假炮制品组、硝苯地平组(阳性对照组)、溶剂组(高血压对照组),n=6;另选10只雄性正常Wistar大鼠作为正常对照组;以给药前后清醒大鼠尾动脉血压、心率、体重为检测指标,分析杜仲各制品的降压活性与特点。结果: ①经各组给药前后进行自身比较,SHR-SP高血压对照组血压、心率较自身分别显著升高29.2 mmHg(P<0.01)、67.7 times·min^-1(P<0.05);硝苯地平、杜仲生药、炮制品均具有显著抑制SHR-SP大鼠自身基础血压、心率升高的活性,杜仲假炮制品则无此活性,杜仲生药与炮制品抑制自身基础血压升高的活性要强于硝苯地平;②经给药后各制品组分别与高血压对照组进行组间比较,硝苯地平组、杜仲生药组、炮制品组血压较高血压对照组分别低47(28%)mmHg、45.1(27%)mmHg、56.3(34%)mmHg,P均<0.01;杜仲假炮制品组血压较高血压对照组无显著性差异(P>0.05);杜仲炮制品组心率较高 血压对照组低47.6 times·min^-1(P<0.05)。结论: 杜仲药材生品与炮制品均有显著降压活性,炮制品降压活性略优于生品,但两者无显著性差异,假炮制品则无降压活性;杜仲炮制品还具有显著降低心率活性。

Objective: To compare the anti-hypertensive activity among different processed materials of Eucommiae Cortex. Methods: A total of 30 male rats with SHR-SP(spontaneously hypertension rat stroke-prone)were used for the study and grouped to equalize pressure values distribution randomly,which included raw material,processed material,false processed material,nifedipine(positive control),and solvent(SHR-SP negative control)groups(n=6).Another 10 male wistar rats were included as normal control group.Blood pressure from tail arteries,heart rate and body weight was measured before and after medication treatment to analyze the anti-hypertensive activity among different processed material of Eucommiae Cortex. Results: ①Self-compared before and after drug administration,the blood pressure and heart rate of SHR-SP negative control rose significantly by 29.2 mmHg(P<0.01),67.7 times·min^-1 (P<0.05)respectively;nifedipine,raw material and processed material all suppressed the automatically elevating blood pressure and heart rate of SHR-SP,which was not found by using false processed material.Their suppressing activity on the automatically elevating blood pressure of raw and processed material was better than that of nifedipine.②Compared with SHR-SP negative control group,the blood pressure after treatment of nifedipine,raw material and processed material groups was significantly lower[47(28%)mmHg,45.1(27%)mmHg,56.3(34%)mmHg,P<0.01];however,there was no significant difference between false processed material group and SHR-SP negative control(P>0.05);in addition,the heart rate of processed material group was significantly lower(47.6 times·min^-1,P<0.05)than that of SHR-SP negative control. Conclusion: Both of the raw material and processed material of Eucommiae Cortex have significant anti-hypertensive activity.The activity of processed material is better than that of raw material but there is no statistical difference.However,the false processed material has no significant anti-hypertensive activity.The processed material of Eucommiae Cortex could also reduce the heart rate.