UPLC-MS/MS法同时检测跌打丸中的6种非法染色物

目的: 建立跌打丸中6种非法染色物的超高效液相色谱-串联质谱检测方法。方法: 样品采用乙醇超声提取,经液相色谱分离,串联四极杆质谱ESI正离子检测,多反应监测(MRM)模式,外标法定量。结果: 6种染色物线性范围为0.01~2.0 μg·mL^-1, 线性相关系数均大于0.996,检测限在0.33~19.50 μg·kg^-1之间,3个浓度水平加标回收率为92.3%~101.6%,RSD为1.6%~3.5%。158批次样品2批次检出苏丹Ⅰ和Ⅳ,2批次检出808猩红。结论: 该方法选择性强,灵敏度和准确度高,可以快速、有效地检测跌打丸中6种非法染色物。

Objective: To develop a UPLC-MS/MS method for the simultaneous determination of six illegal dyes in Dieda pills. Methods: Samples were extracted by ethanol.The analytes were then separated by UPLC and detected by multi-reactions monitoring(MRM)technology of tandem mass spectrometry with electrospray ionization source. Results: The calibration curves of six dyes showed good linearity in the range of 0.01-2.0 μg·mL^-1 and correlation coefficients were all above 0.996.The lower limits of determination for six dyes in samples were between 0.33 and 19.5 μg·kg^-1.The average recoveries at three levels ranged from 92.3%-101.6%with RSD of 1.6% to 3.5%.Sudan Ⅰ and sudan Ⅳ could be detected in 2 of 158 samples, and 808 scarlet could be detected in another 2 samples. Conclusion: The established method is selective, sensitive and accurate, which is suitable for the supervision and determination of six illegal dyes in Dieda pills.

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