HPLC法同时测定淫羊藿-川芎药对8种化学成分的含量

目的: 建立补肾活血药对淫羊藿-川芎中淫羊藿苷、朝藿定A、朝藿定B、朝藿定C、宝藿苷-I、川芎嗪、阿魏酸和藁本内酯8种成分的HPLC含量测定方法,明确配伍对8种成分含量变化的影响。方法: 采用RP-HPLC多波长切换梯度洗脱技术。色谱柱:Waters X Bridge-C₁₈(4.6 mm×250 mm,5 μm);流动相:乙腈-1%醋酸梯度洗脱;流速:1.0 mL·min^-1;分别以各成分最大吸收波长为检测波长;柱温:30 ℃。结果: 淫羊藿-川芎药对中淫羊藿苷、川芎嗪、阿魏酸等8成分均呈良好的线性关系(r≥0.999);平均加样回收率(n=6)为97.7%~103.8%(RSD<3%,n=6)。淫羊藿、川芎配伍后川芎嗪、阿魏酸、藁本内酯的含量分别由0.714、2.701、12.930 mg·g^-1变成1.492、3.032、13.97 mg·g^-1,含量显著增加,而淫羊藿苷、朝藿定A、朝藿定B、朝藿定C和宝藿苷-I的含量分别由5.190、1.762、2.423、1.965、0.712 mg·g^-1变成5.475、1.645、2.440、2.035、0.729 mg·g^-1,含量则无明显变化。结论: 该方法简便快速,结果准确可靠,方法重复性好,可用于淫羊藿-川芎药对及其制剂的质量控制,并为淫羊藿、川芎的临床配伍应用及其研究提供科学依据。

Objective: To establish an HPLC method of simultaneous determination of 8 indicative components(icariin,epimedin A,epimedin B,epimedin C,baohuoside I,tetramethylpyrazine,ferulic acid and ligustilidein)in Epimedii Folium and Chuanxiong Rhizoma,and investigate the effects of compatibility of Chuanxiong Rhizoma with Epimedii Folium on content changes of icariin,epimedin A,epimedin B,epimedin C,baohuoside I,tetramethylpyrazine,ferulic acid and ligustilidein. Methods: RP-HPLC and the gradient elution wavelength shift technique was used in the study,where the column was Waters X Bridge-C₁₈ (4.6 mm×250 mm,5 μm),the mobile phase was acetonitrile-1% acetic acid solution,the flow rate was 1 mL·min^-1,the detection wavelength was the maximum absorption wavelength of each ingredient,and the oven temperature was 30 ℃. Results: The 8 indicative components had good linearity(r≥0.999).The average recoveries were 97.7%-103.8%(RSD<3%,n=6).The contents of tetramethylpyrazin,ferulic acid and ligustilidein in Chuanxiong Rhizoma changed from 0.714 mg·g^-1,2.701 mg·g^-1 and 12.930 mg·g^-1 to 1.492 mg·g^-1,3.032 mg·g^-1 and 13.97 mg·g^-1 respectively,which increased significantly.The contents of icariin,epimedin A,epimedin B,epimedin C,and baohuoside I in Epimedii Folium changed from 5.190 mg·g^-1,1.762 mg·g^-1,2.423 mg·g^-1,1.965 mg·g^-1 and 0.712 mg·g^-1 to 5.475 mg·g^-1,1.645 mg·g^-1,2.440 mg·g^-1,2.035 mg·g^-1 and 0.729 mg·g^-1 respectively,which did not change significantly after compatibility of Chuanxiong Rhizoma with Epimedii Folium. Conclusion: This method is simple,repeatable and rapid.The results are accurate and reliable,indicating that the method can be used to control the quality of Epimedii Folium and Chuanxiong Rhizoma and their preparations,and provide the scientific basis for the clinical compatibility of Epimedii Folium and Chuanxiong Rhizoma and the related research.