HPLC法同时测定不同产地车前子生品及盐炙品中3个主要活性成分的含量

目的: 建立高效液相色谱法同时测定不同产地车前子生品及盐炙品中车前素、毛蕊花糖苷和异毛蕊花糖苷3个主要活性成分的含量。方法: 采用CAPCELL PAK C₁₈ MG Ⅱ S5色谱柱(250 mm×4.6 mm,5 μm),以甲醇(A)-水(B)为流动相,梯度洗脱(0~45 min,25%A→42%A),流速1.0 mL · min^-1,检测波长210 nm,柱温30 ℃。结果: 车前素、毛蕊花糖苷和异毛蕊花糖苷进样量分别在0.045~0.898 μg(r=0.999 9)、0.212~4.234 μg(r=0.999 9)和0.017~0.338 μg(r=1.000 0)范围内与峰面积呈现良好的线性关系;平均回收率(n=6)分别为99.3%、100.0%和99.1%,RSD分别为0.52%、0.18%和1.9%。结论: 该方法分离效率高,重复性好,经方法学验证,可用于车前子药材的质量控制。

Objective: To establish an HPLC method to simultaneously determine three main kinds of active constituents, namely, plantagoguanidinic acid, verbascoside and isoverbascoside, before and after salt processing of Plantaginis Semen from different habitats. Methods: The CAPCELL PAK C₁₈ MG Ⅱ S5 column(250 mm×4.6 mm, 5 μm)was used with the mobile phase of methonal(A)-water(B)for gradient elution(0-45 min, 25%A→42%A)at the flow rate of 1.0 mL · min^-1.The detection wavelength was 210 nm, and the column temperature was at 30℃. Results: The linear ranges of plantagoguanidinic acid, verbascoside and isoverbascoside were 0.045-0.898 μg(r=0.999 9), 0.212-4.234 μg(r=0.999 9)and 0.017-0.338 μg(r=1.000 0), respectively. The average recoveries(n=6)were 99.3%(RSD=0.52%), 100.0%(RSD=0.18%)and 99.1%(RSD=1.9%), respectively. Conclusion: Based on method validation, this method with high separation efficiency and good repeatability can be used for the quality control of Plantaginis Semen.

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