微波消解-电感耦合等离子体质谱法测定大鼠全血中纳米银

目的: 建立和确证一种简便、准确的测定大鼠全血中银浓度的分析方法。方法: 选取铟(¹¹⁵In)为内标,先用硝酸对全血样品进行微波消解处理,再采用电感耦合等离子体质谱法测定消化液中银离子的浓度。结果: 内源性物质不干扰银和内标的测定,全血中银的浓度在5.0~1 000 μg · L^-1范围内线性关系良好,日内精密度RSD≤3.3%,日间精密度RSD≤3.8%;提取回收率为97.5%~101.9%,基质效应为97.4%~99.4%。应用该法研究了大鼠静脉注射纳米银后银的体内经时变化,结果表明,给药(40 nm)后,大鼠血液中银浓度先迅速下降而后上升,之后再缓慢消除。结论: 该方法可为纳米银在生物机体内的评价研究提供分析方法参考。

Objective: To develop a simple and accurate analytical method for the determination of silver in rat whole blood. Methods: Using¹¹⁵In as internal standard, whole blood samples were digested by microwave digestion with nitric acid, followed by ICP-MS analysis to determine the concentration of silver ion. Results: No significant endogenous interferences were detected.The calibration curve was linear within the range of 5.0-1 000 μg · L^-1.The intra-day and inter-day precisions were no more than 3.3%and 3.8%, respectively.The extraction recoveries were within 97.5%-101.9% and the matrix effects were within 97.4%-99.4%.The method was applied to the pharmacokinetic study of silver in rats after intravenous administration of silver nanoparticles and the results showed that the silver concentration was rapidly decreased then increased and then slowly eliminated. Conclusion: The method could provide reference for the future work aiming to in vivo assessment of silver nanoparticles.

[1] HE W(何伟), ZHANG FM(张发明), FAN ZN(范志宁).Application of nano-silver antibacterial technology in clinical healthcare(纳米银抗菌技术在临床医疗中的应用)[J].J Clin Rehabil Tissue Eng Res(中国组织工程研究与临床康复), 2010, 14(42):7899
[2] CHEN J(陈炯), HAN CM(韩春茂), LIN XW(林小玮), et al.Effect of silver nanoparticles dressing on second degree burn wound(纳米银敷料在修复二度烧伤创面的应用研究)[J].Chin J Surg(中华外科杂志), 2006, 44(1):50
[3] XUE YY(薛玉英), TANG M(唐萌).Research progress in biological effects of nanosilver(纳米银生物学效应研究)[J].J Southeast Univ:Nat Sci Ed(东南大学学报:自然科学版), 2009, 39(6):1316
[4] LANKVELD DPK, OOMEN AG, KRYSTEK P, et al.The kinetics of the tissue distribution of silver nanoparticles of different sizes[J].Biomaterials, 2010, 31(32):8350
[5] PARK K, PARKV, CHUN IK, et al.Bioavailability and toxicokinetics of citrate-coated silver nanoparticles in rats[J].Arch Pharm Res, 2011, 34(1):153
[6] REN HX(任红心), YU T(于彤), LI XT(李晓天), et al.Distribution of nanometer antibacteraial gel in main organ of rats(纳米银抗菌凝胶在大鼠体内主要脏器的分布)[J].J Med Forum(医药论坛杂志), 2013, 34(1):36
[7] U.S.Department of Health and Human Services, Food and Administration Center for Drug Evaluation and Research(CDER), Center for Veterinary Medicine(CVM).Guidance for Industry:Bioanalytical Method Validation [DB/OL].2001-05[2007-9-12].http://www.fda.gov/downloads/drugs/guidancecomplianceregulatoryinformation/guidances/ucm070107.pdf
[8] ZHONG DF(钟大放).The research of establishing standard curves of biochenmical analysis with weighted least square method(以加权最小二乘法建立生物分析标准曲线的若干问题)[J].Chin J Pharm Anal(药物分析杂志), 1996, 16(1):343
[9] ZHANG SS(张姗姗), XUE YY(薛玉英), TANG M(唐萌).Biodistribution of nanosilvers in mice(纳米银在小鼠体内的组织分布)[J].J Southeast Univ:Nat Sci Ed(东南大学学报:自然科学版), 2012, 42(2):388
[10] KRYSTEK P.A review on approaches to bio distribution studies about gold and silver engineered nanoparticles by inductively coupled plasma mass spectrometry[J].Microchem J, 2012, 105:39
[11] TIAN BZ(田宝珍), CAO FC(曹福苍), LEI PJ(雷鹏举), et al.Dissolution of biological samples by microwave digestion for the determination of trace elements(微波消解制样技术用于生物样品微量分析的研究)[J].Food Ferment Ind(食品与发酵工业), 1999, 26(3):15