HPLC法测定芪胶升白胶囊中朝藿定C和淫羊藿苷的含量

目的: 建立中成药芪胶升白胶囊中朝藿定C和淫羊藿苷的含量测定方法。方法: 采用高效液相色谱法,使用Diamonsil C₁₈色谱柱(250 mm×4.6 mm,5 μm),以乙腈-水(24.5: 75.5)为流动相,流速1 mL·min^-1,检测波长270 nm,柱温25 ℃。结果: 朝藿定C进样量在0.027~0.542 μg范围内线性良好(r=0.999 8),淫羊藿苷进样量在0.025~0.499 μg范围内线性良好(r=0.999 9)。朝藿定C的平均回收率(n=6)为99.27%,RSD为1.2%;淫羊藿苷的平均回收率(n=6)为100.2%,RSD为1.6%。测得3批次芪胶升白胶囊中的朝藿定C、淫羊藿苷的平均含量范围为0.570~0.599 mg·g^-1、0.395~0.409 mg·g^-1。结论: 本方法经方法学验证,可作为芪胶升白胶囊中朝藿定C和淫羊藿苷含量测定的方法。

Objective: To establish a method for determination of epimedin C and icariin in Chinese patent medicine Qijiaoshengbai capsules. Methods: HPLC was used with the Diamonsil C₁₈ (250 mm×4.6 mm,5 (] 75.5)as the mobile phase at a flow rate of 1.0 mL·min^-1,the detecting wavelength of 270 nm,and the column temperature at 25 ℃. Results: The good linear was over the range of 0.027-0.542 μg for epimedin C (r=0.999 8)and the average recovery (n=6)was 99.27% with RSD of 1.2%;the good linear was over the range of 0.025-0.499 μg for icariin (r=0.999 9)and the average recovery (n=6)was 100.2% with RSD of 1.6%.The content ranges in three batches of Qijiaoshengbai capsules were 0.570-0.599 mg·g^-1 for epimedin C and 0.395-0.409 mg·g^-1 for icariin. Conclusion: This validated method can be used for the determination of epimedin C and icariin in Qijiaoshengbai capsules.