HPLC法同时测定绿衣枳壳中3种黄酮类化合物的含量

目的: 建立HPLC法同时测定绿衣枳壳中柚皮苷、橙皮苷及枳属苷的含量。方法: 采用Hypersil ODS2(250 mm×4.6 mm,5 μm)色谱柱,以乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱(0~15 min,20%A;15~17 min,20%A→30%A;17~27 min,30%A),流速1.0 mL·min^-1,检测波长283 nm。结果: 柚皮苷、橙皮苷及枳属苷进样量分别在0.080~2.667、0.033~1.088、0.090~3.013 μg范围内呈良好线性关系(r=0.999 6~0.999 9);各组分平均回收率(n=3)在99.63%~101.5%之间,RSD为0.040%~1.2%。10批样品测定结果为柚皮苷含量1.62%~2.02%,橙皮苷含量0.045%~0.063%,枳属苷含量1.66%~1.90%。结论: 本法可用于同时测定绿衣枳壳中柚皮苷、橙皮苷及枳属苷的含量。

Objective: To develop an HPLC method to simultaneously determine naringin,hesperitin and poncirin in Fructus Ponciri Trifoliatae Immaturi. Methods: The Hypersil ODS2(250 mm×4.6 mm,5 μm) column was used.Acetonitrile(A)-0.1% phosphoric acid(B)was used as the mobile phase with gradient elution(0-15 min,20%A;15-17 min,20%A→30%A;17-27 min,30%A),at a flow rate of 1.0 mL·min^-1.The detection wavelength was 283 nm. Results: The calibration curves of naringin,hesperitin and poncirin were in good linearity over 0.080-2.667 μg,0.033-1.088μg,0.090-3.013μg(r=0.999 6-0.999 9).The average recovery was in the range of 99.63%-101.5% and RSD was in the range of 0.040%-1.2%.The contents in 10 samples were in the range of 1.62%-2.02% for naringin,0.045%-0.063% for hesperidin and 1.66%-1.90% for poncirin. Conclusion: The method can be used to determine the contents of naringin,hesperitin and poncirin in Fructus Ponciri Trifoliatae Immaturi.

[1] State Administration of Traditional "Chinese Materia Medica" Editorial(国家中医药管理局《中华本草》编委会).Chinese Materia Medica(中华本草)[M].Shanghai(上海):Shanghai Science and Technology Publishers(上海科学技术出版社),1996:880
[2] HUANG YL(黄有霖).Chinese Materia Medica Standards Fujian Province(福建省中药材标准)[M].Fuzhou(福州):Sea Breeze Publishing(海风出版社),2006:231
[3] ZHANG B(章斌),JIN J(金剑).Pharmacological effects and clinical application of Citrus aurantium(枳壳的药理作用与临床应用进展)[J].Her Med(医药导报),2013,32(11):1462
[4] PAN X(潘馨).Determination of naringin in Fructus Ponciri Trifoliatae Immaturi by HPLC(绿衣枳壳中柚皮苷含量测定方法的研究)[J].Cent South Pharm(中南药学),2005,3(2):82
[5] LI H(李煌).Determination of naringin in Fructus Ponciri Trifoliatae Immaturi collected in different seasons(不同采收期绿衣枳壳中柚皮苷的含量测定)[J].Chin J Inf Tradit Chin Med(中国中医药信息杂志),2008,15(7):43
[6] PAN X(潘馨).Studies on the fingerprint of Fructus Ponciri Trifoliatae Immaturi(绿衣枳壳指纹图谱的研究)[J].Strait Pharm J(海峡药学),2003,15(6):40
[7] BIAN QQ(边清泉),LIU SM(刘思曼),YANG ZP(杨振萍).Determination of hesperidin and naringin in orange,orange and naringin(橘、橙、柚皮中橙皮苷和柚皮苷含量的测定)[J].Chem Res Appl(化学研究与应用),2008,20(11):1523
[8] LI XL(李秀玲),LI L(李龙),XIAO HB(肖红斌),et al.Determination of hesperidin and naringin in Fructus Aurantii Immaturus and Fructus Aurantii(反相高效液相色谱法测定枳实、枳壳中橙皮甙和柚皮甙的含量)[J].Chin J Chromatogr(色谱),2002,20(6);585
[9] XIE ZJ(谢贞建),JIAO SR(焦士蓉),LI K(李恺),et al.Determination of naringin,hesperidin and neohesperidin content in Frutus Aurantii Immaturus from different places by RP-HPLC approach(RP-HPLC法测定不同产地枳实中的柚皮苷、橙皮苷及新橙皮苷).J Xihua Univ:Nat Sci(西华大学学报:自然科学版),2009,28(2):65
[10] WANG Q(王青).Determination of flavonoids in Fructus Aurantii Immaturs and Fructus Aurantii from different habitat by HPLC(不同产地枳实与枳壳中黄酮类成分的含量测定)[J].Heilongjiang Med J(黑龙江医药),2008,21(3):1