HPLC法测定杞菊地黄丸中莫诺苷、马钱苷和丹皮酚的含量

目的: 建立一种快速、准确和实用的HPLC方法,用于同时测定杞菊地黄丸中莫诺苷、马钱苷和丹皮酚的含量。方法: 采用Phenomenex Gemini C₁₈ (4.6 mm×250 mm,5 μm)色谱柱,以乙腈-0.3%磷酸水溶液为流动相梯度洗脱,流速1.0 mL · min^-1,柱温40 ℃,检测波长为240 nm(莫诺苷、马钱苷)和274 nm(丹皮酚)。结果: 莫诺苷、马钱苷、丹皮酚质量浓度分别在2.242~44.84 μg · mL^-1 (r=0.999 9)、2.130~42.60 μg · mL^-1 (r=1.000)、5.068~101.4 μg · mL^-1 (r=1.000)范围内,与峰面积线性关系良好;平均回收率分别为96.2%(RSD=0.44%)、96.0%(RSD=0.21%)、103.0%(RSD=0.65%)。结论: 本方法可作为杞菊地黄丸中莫诺苷、马钱苷和丹皮酚含量同时测定的方法,适用于杞菊地黄丸的质量控制。

Objective: To establish a rapid, accurate and feasible HPLC method for simultaneous determination of morroniside, loganin and paeonol in Qiju Dihuang pills. Methods: The separation was carried out on a Phenomenex Gemini C₁₈ (4.6 mm×250 mm, 5 μm)column, the mobile phase consisting of 0.3% phosphoric acid solution(B)-acetonirtile(A)at a flow rate of 1.0 mL · min^-1, with the column temperature of 40 ℃, the detection wavelength at 240 nm for morroniside and loganin and 274 nm for paeonol. Results:The calibration curve was liner over the range of 2.242-44.84 μg · mL^-1 (r=0.999 9)for morroniside, 2.130-42.60 μg · mL^-1 (r=1.000)for loganin and 5.068-101.4 μg · mL^-1 (r=1.000)for paeonol.The average recovery of the three components was 96.2%(RSD=0.44%), 96.0%(RSD=0.21%), and 103.0%(RSD=0.65%), respectively. Conclusion:The method can be used for simultaneous determination of morroniside, loganin and paeonol in Qiju Dihuang pills, and it is applicative applicable for quality control of Qiju Dihuang pills.

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