HPLC法同时测定玉叶清火胶囊中栀子苷、穿心莲内酯和脱水穿心莲内酯
Simultaneous determination of geniposide,andrographolide and dehydroandrographolide in Yuye Qinghuo capsules by HPLC
分类号：
出版年·卷·期（页码）：2015,35 (2):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的:建立玉叶清火胶囊中栀子苷、穿心莲内酯和脱水穿心莲内酯含量的液相色谱测定法。方法:采用Waters SunFireTM C18 色谱柱(4.6 mm×150 mm,5 μm),以甲醇-0.2%磷酸水溶液为流动相,梯度洗脱,流速1.0 mL · min-1,检测波长为切换波长(0~10 min,238 nm,栀子苷;10~22 min,225 nm,穿心莲内酯;22~35 min,254 nm,脱水穿心莲内酯)。结果:栀子苷进样量在0.148~2.659 μg范围内线性关系良好(r=0.999 9,n=6),平均回收率(n=9)为99.90%;穿心莲内酯进样量在0.149~2.681 μg范围内线性关系良好(r=0.999 8,n=6),平均回收率(n=9)为99.62%;脱水穿心莲内酯进样量在0.089~1.607 μg范围内线性关系良好(r=0.999 9,n=6),平均回收率(n=9)为99.53%。结论:本文建立的波长切换高效液相色谱法简便、快捷,可用于该制剂的质量检测。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To establish an HPLC method for the determination of geniposide, andrographolide and dehydroandrographolide in Yuye Qinghuo capsules. Methods: The Waters SunFireTM C18 (4.6 mm×150 mm, 5 μm)column was adopted.The mobile phase was methanol-water containing 0.2% phosphoric acid with gradient elution mode at the flow rate of 1.0 mL · min-1;the detection wavelength was 238 nm(0-10 min)for geniposide, 225 nm(10-22 min)for andrographolide and 254 nm(22-35 min)for dehydroandrographolide. Results: Good linearity was shown in the range of 0.148-2.659 μg for geniposide with the average recovery(n=9)of 99.90%, 0.149-2.681 μg for andrographolide with the average recovery(n=9)of 99.62%, 0.089-1.607 μg for dehydroandrographolide with the average recovery(n=9)of 99.53%. Conclusion: The wavelength switching-high performance liquid chromatography method is simple, rapid and convenient, which can be used for quality detection of Yuye Qinghuo capsules.
-----参考文献：---------------------------------------------------------------------------------------
[1] LI K(李柯).Determination of andrographolide and dehydroandrographolide in Yuyeqinghuo capsule with HPLC(HPLC法测定玉叶清火胶囊中穿心莲内酯和脱水穿心莲内酯的含量研究)[J].Guid J Tradit Chin Med(中医药导报), 2007, 13(10):68
[2] GONG HN(龚海南).Determination of geniposide in Yuye Qinghuo Jiaonang by HPLC(高效液相色谱法测定玉叶清火胶囊中京尼平苷的含量)[J].Cent South Pharm(中南药学), 2010, 8(6):448
[3] ChP 2010.Vol Ⅰ(中国药典2010年版.一部)[S].2010:231, 251
[4] GUO M(郭敏).HPLC determination of dehydroandrographolide and geniposide in Qinghuozhimai capsules(HPLC法同时测定清火栀麦胶囊中脱水穿心莲内酯和栀子苷的含量)[J].Chin J Pharm Anal(药物分析杂志), 2008, 28(11):1921
[5] LAN XY(蓝晓玉), LIN H(林华).HPLC determination of three effective components in Qinghuo Zhimai tablets(HPLC法同时测定清火栀麦片中3种有效成分的含量)[J].Chin J Pharm Anal(药物分析杂志), 2011, 31(2):228
欢迎阅读《药物分析杂志》！您是该文第 836位读者！
