GC法同时测定复方牙痛酊中樟脑和乙酸龙脑酯的含量

目的: 建立GC法同时测定复方牙痛酊中樟脑和乙酸龙脑酯的含量. 方法: 采用DB-WAX毛细管柱(30 m×0.25 mm×0.25 μm) ,氢火焰离子检测器(FID);以萘为内标;进样口温度230 ℃,检测器温度250 ℃,程序升温(初始温度105 ℃,保持3 min,以3 ℃·min^-1的速率升至140 ℃,再以100 ℃·min^-1的速率升至210 ℃,保持5 min),分流进样,分流比2.0:1,进样量1 μL. 结果: 樟脑、乙酸龙脑酯浓度分别在1.81~27.18 μg·mL^-1和5.53~82.95 μg·mL^-1范围内呈良好的线性关系(r=0.999 9),平均回收率(n=9)分别为103.9%和105.2%. 结论: 本方法可用于测定复方牙痛酊中樟脑和乙酸龙脑酯的含量.

Objective: To establish a GC method for simultaneous determination of contents of camphor and borneol acetate in compound Yatong tincture. Methods: A DB-WAX capillary column (30 m×0.250 mm×0.25 μm) and a FID were adopted. Naphthalene was used as an internal standard substance. The injector temperature was 230 ℃ and the detector temperature was 250 ℃. The initial temperature was 105 ℃, maintained for 3 min, rose to 140 ℃ with a rate of 3 ℃·min^-1, then rose to 210 ℃ with a rate of 100 ℃·min^-1, maintained for 5 minutes. The split ratio for split stream sampling was 2.0:1. The injection volume was 1 μL. Results: The linear ranges of camphor and borneol acetate were 1.81-27.18 μg·mL^-1(r=0.999 9) and 5.53-82.95 μg·mL^-1 (r=0.999 9), respectively. Their average recovery rates (n=9) were 103.9% and 105.2%, respectively. Conclusion: The developed method is suitable for the determination of camphor and borneol acetate in compound Yatong tincture.

[1] WAN HC(万呼春),ZHANG S(张爽),WU NY(吴妮娅),et al.Clinical effects of Fufang Yatongding on gingivitis and pericoronitis(复方牙痛酊对牙龈炎、冠周炎临床治疗效果观察) [J].West China J Stomatol(华西口腔医学杂志),2008,26(2):162
[2] State Drug Administration(国家药品监督管理局). The National Chinese Medicine Standard Collection of Oral Tumor Pediatric Fascicle(国家中成药标准汇编口腔肿瘤儿科分册) [S].2002:300
[3] Shanghai Chemical Reagent Co.,Ltd, Sinopharm Group(中国医药集团上海化学试剂公司). Handbook of Reagents(试剂手册) [M].3rd ed(第三版).Shanghai(上海):Shanghai Scientific and Technical Publishers(上海科学技术出版社),2002:247
[4] SONG LR(宋立人), HONG X(洪恂), DING XL(丁绪亮), et al. Contemporary Traditional Chinese Medicine Lexicon(现代中药大辞典) [M].Beijing(北京):People’s Medical Publishing House(人民卫生出版社),2001:2400
[5] YANG Q(杨乾), JU AH(鞠爱华),BAI WF(白万富),et al. The research advance in chemical constituents and pharmacology-activity for Valeriana officinalis Linn. var. latiofolia Miq.(宽叶缬草的化学成分及药理活性研究进展) [J].Chin J Mod App Pharm(中国现代应用药学),2008,25(z1):613
[6] DU XW(都晓伟),WU JK(吴军凯).The research advance in chemical constituents and pharmacology-activity for the Valeriana L.(缬草属植物化学成分及药理活性研究进展) [J]. World Phytomed(国外医药植物药分册),2006,21(1):10
[7] ChP 2010. VolⅠ(中国药典2010年版.一部) [S].2010:421,931,958
[8] JIANG LY(蒋玲燕),LI Q(李清),CHEN XH(陈晓辉),et al. GC simultaneous determination of four components in Houttuynia cordata injection(GC法同时测定鱼腥草注射液中4种成分的含量) [J].Chin J Pharm Anal(药物分析杂志),2011,31(4):664
[9] SUN YQ(孙毓庆).Analytical Chemistry(分析化学) [M].Beijing(北京):People’s Medical Publishing House(人民卫生出版社),1999:263