HPLC法测定多叶越南槐中红车轴草苷及高丽槐素的含量

目的: 建立测定多叶越南槐药材中红车轴草苷及高丽槐素2个黄酮类成分含量的高效液相色谱法. 方法: 采用Agilent Eclipse XDB-C₁₈色谱柱(4.6 mm×250 mm,5 μm),柱温25 ℃,以乙腈-0.1%乙酸水溶液为流动相,梯度洗脱,流速1.0 mL·min^-1, 检测波长310 nm,进样量10 μL,外标法定量. 结果: 红车轴草苷进样量在0.025 1~2.51 μg范围内与峰面积呈良好的线性关系(r=0.999 0),平均回收率为97.0%(RSD=1.5%);高丽槐素进样量在0.025 2~2.52 μg范围内与峰面积呈良好的线性关系(r=0.999 0),平均回收率为101.3%(RSD=2.2%). 结论: 建立的含量测定方法符合方法验证要求.

Objective: To establish an HPLC method to determine the content of two flavonoids(trifolirhizin and maackiain)in Sophora tonkinensis Gagnep.var.polyphylla S.Z.Huang et Z.C.Zhou. Methods: An Agilent Eclipse XDB-C₁₈ column(4.6 mm×250 mm,5 μm)was adopted,and the mobile phase consisted of acetonitrile and 0.1% aqueous acetic acid with a gradient elution.The flow rate was 1.0 mL·min^-1,the column temperature was 25 ℃,the detection wavelength was 310 nm and the injection volume was 10 μL,the sample was quantified with external standard. Results: The calibration curve of trifolirhizin was in good linearity at 0.025 1-2.51 μg (r=0.999 0)and the average recovery was 97.0% with RSD of 1.5%.The calibration curve of maackiain was in good linearity at 0.025 2-2.52 μg(r=0.999 0)and the average recovery was 101.3% with RSD of 2.2%. Conclusion: The method established in this paper conforms to the requirements of the methodology validation.