表面解吸常压化学电离质谱快速分析人参花中挥发性成分

目的: 建立新型表面解吸常压化学电离(surface desorption atmospheric pressure chemical ionization,SDAPCI)质谱法对人参花蕾中挥发性成分的快速鉴别.方法: 采用SDAPCI质谱法,无需色谱分离,对水蒸气蒸馏法获得的人参花挥发油进行直接检测.在空气相对湿度为50%的敞开体系下,通过电晕放电,在正离子模式可产生以H₃O⁺为主的初级离子,使得挥发成分与之结合产生[M+H]⁺而被检测.结果: 通过该方法共检测出34个化合物,通过串联质谱(MS/MS)分析结合气相色谱-质谱(GC-MS)数据比对,鉴定出其中10个挥发性成分.结论: 实验结果表明,与GC-MS相比,该方法快捷灵敏,无污染,不需要样本制备,可广泛应用于实时及在线检测复杂基质中挥发性成分的鉴定.

Objective: To establish a method of surface desorption atmospheric pressure chemical ionization(SDAPCI)-mass spectrometry(MS)for identification of the volatile components of Ginseng Flos. Methods: Through the SDAPCI approach, the direct detection was carried out for the volatile oil which was gained by a steam distillation method without any chromatographic separation.An in-house corona discharge was employed to generate the primary ions such as H₃O⁺ which was combined with the volatile compounds to generate [M+H]⁺ for mass analysis in the positive ion detection mode in the ambient air with 50% relative humidity. Results: Through this method, 34 compounds were detected, of which 10 were identified as the volatile oil from Ginseng Flos. Conclusion: Compared with the results of gas chromatography(GC)-mass spectrometry(MS), SDAPCI-MS is more rapid, sensitive and sample-preparation-free for analysis of volatile components of the plants, which can be used for real time and online detection of various compounds in complex matrices including volatile samples.