RP-HPLC同时测定杨树花和杨树叶中3个植物甾醇含量

建立一种同时测定杨树花和杨树叶中豆甾醇、麦角甾醇、β-谷甾醇含量的反相高效液相色谱法.方法: 以甲醇为流动相,测定杨树花和杨树叶中豆甾醇、麦角甾醇、β-谷甾醇的含量.结果: 杨树花和杨树叶中3个植物甾醇的线性范围为0.00008~0.08 μmol·mL^-1,r>0.9990.杨树花中麦角甾醇、豆甾醇、β-谷甾醇的加样回收率分别在92.6%~100.0%、93.9%~100.1%、94.0%~100.0%之间,RSD(n=6)分别为2.8%、2.2%、2.8%;杨树叶中各甾醇加样回收率分别在91.3%~100.0%、91.2%~100.0%、92.5%~100.1%之间,RSD(n=6)分别为2.7%、2.7%、2.5%.结论: 该方法稳定、快速,精密度高,线性范围宽,适合杨树花和杨树叶中的豆甾醇、麦角甾醇、β-谷甾醇含量测定.

Objective: To establish a method for determination of the contents of three phytosterols(stigmasterol, ergosterol, β-sitosterol)from Flos Populi Canadensis and leaves of Populus canadensis Moench by RP-HPLC. Methods: With methanol as the mobile phase, HPLC method was adopted to determine contents of phytosterols in Flos Populi Canadensis and leaves of Populus canadensis Moench. Results: The three phytosterols had good linearity in their ranges of 0.00008-0.08 μmol·mL^-1(the coefficients of correlation were all over 0.9990).The average recoveries of stigmasterol, ergosterol, β-sitosterol in Flos Populi Canadensis were 92.6%-100.0%, 93.9%-100.1%, 94.0%-100.0%, and RSDs(n=6)were 2.8%, 2.2%, 2.8%, respectively;the average recoveries(n=6)of the three phytosterols in leaves of Populus canadensis Moench were 91.3%-100.0%, 91.2%-100.0%, 92.5%-100.1%, and RSDs were 2.7%, 2.7%, 2.5%, respectively. Conclusion: The method is sensitive, accurate and reproducible, and reliable for the determination of phytosterols in Flos Populi Canadensis and leaves of Populus canadensis Moench.

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