溴化十六烷基三甲铵琼脂培养基促生长能力的评价分析

目的: 通过对铜绿假单胞菌在溴化十六烷基三甲铵琼脂培养基中生长性能差异的分析,建立培养基的评价分析方法. 方法: 利用96孔板设置不同培养基处方(3种胨源×2种培养基配方)、溴化十六烷基三甲铵浓度(6水平)和铜绿假单胞菌浓度(4水平)的组合,分析在各种不同的组合条件下铜绿假单胞菌的生长情况. 结果: 不同溴化十六烷基三甲铵琼脂促生长能力的差异主要由胨源和配方所决定;USP/EP收载的溴化十六烷基三甲铵琼脂配方优于目前中国药典收载的配方. 结论: 多因素多水平的微量分析方法可较好地揭示影响培养基促生长能力的诸因素,为培养基配方的筛选开拓新思路.

Objective: To develop a novel method for evaluation of the medium by analyzing differences in the growth property of Pseudomonas aeruginosa on cetrimide agar. Methods: Combinations of different media(3 peptones×2 medium formulas)with cetrimide concentrations(6 levels)and Pseudomonas aeruginosa concentrations(4 levels)were set up on the 96-well plate.And the growth promoting property of Pseudomonas aeruginosa under different combination conditions was analyzed. Results: Peptone source and medium formula were the main determinants for the growth property differences of Pseudomonas aeruginosa on cetrimide agar.The cetrimide agar formula in USP/EP was better than that in ChP 2010. Conclusion: Trace analysis with multiple factors and multiple levels could well exhibit all the factors referring to the growth promotion property of medium and provide new thoughts for the selection of medium formula.

[1] ChP 2010. Vol Ⅱ(中国药典2010年版.二部)[S].2010:Appendix(附录)ⅪJ 114
[2] USP 34-NF 29.Vol Ⅰ[S].2011:61
[3] EP 7.0[S].2011:171
[4] CHEN Tian-shou(陈天寿).Manufacture and Application of Microbiological Medium(微生物培养基的制造与应用)[M].Beijing(北京):China Agriculture Press(中国农业出版社),1995:70
[5] JI Shao-mei(纪绍梅).Quality Control and Illustration of Microbiological Medium(微生物培养基质控与图解)[M].Beijing(北京):Science Press(科学技术出版社),2006:4,133
[6] ChP 2010.Vol Ⅱ(中国药典2010年版.二部)[S].2010:Appendix(附录)ⅪJ 111
[7] CHEN Fen(陈芬),ZHANG Xiao-yong(张晓勇),ZHONG Juan(钟娟), et al.Establishment of a 96-well microplate screening method for high yield strains of Jiena-peptide(捷安肽素高产突变株96孔板筛选方法的建立)[J].Biotechnol Bull(生物技术通报),2010(3):204
[8] CAI Wan-hua(蔡万华),DING Shao-peng(丁少鹏),YE Rui-fang(叶蕊芳), et al.Compound screening high-yield Pachysolen tannophilus by use of 96-well plateand growth cycle of Saccharomyces cerevisiae(利用96孔板和酿酒酵母生长圈符合筛选高产管囊酵母)[J].Liquor-making Sci Technol(酿酒科技),2011(4):36
[9] JIA Jun-ping(贾俊平).Fundermental Statistics(统计学基础)[M].Beijing(北京):China Renmin University Press(中国人民大学出版社),2010:139
[10] ZHANG Chun-hua(张春华).Medical Mathematical Statistics(医药数理统计)[M].Beijing(北京):Science Press(科学技术出版社),2001:126
[11] LI Hui-zhen(李会珍),SUN Zi-wen(孙子文),LI Xiao-jun(李晓君), et al.Correlation analysis of seed traits and nutritional components in Perilla(紫苏种子性状与主要营养成分相关性分析)[J].J Chin Cereal Oil Assoc(中国粮油学报),2013,28(10):55
[12] XU Cong(徐丛),XU Shi-tian(徐世田),WANG Hong-bin(汪宏斌), et al.Relationship between BMD and biomechanics of femoral head in human(DEXA测量松质骨BMD与其生物力学相关性). Chin J Osteoporos(中国骨质疏松杂志),2007,13(3):152
[13] TANG Dao-bang(唐道邦),ZHANG You-sheng(张友胜),XU Yu-juan(徐玉娟), et al.Relevance analysis among indexes of grain weight,pulp water activity and microbial of dried litchi(荔枝干颗粒重与果肉水分活度、微生物等指标相关性分析). Guangdong Agric Sci(广东农业科学),2010,37(10):29