RP-HPLC法同时测定枫香树叶药材中3个主要黄酮苷成分的含量

目的： 建立同时测定枫香树叶药材中杨梅苷、异槲皮苷和山柰酚-4'-O-β-D-吡喃葡萄糖苷含量的高效液相色谱法。 方法： 采用YMC-Pack ODS-A色谱柱（150 mm×4.6 mm，5 μm），以乙腈-0.2%磷酸水溶液（13：87）为流动相，流速1.0 mL·min^-1，检测波长351 nm，柱温25 ℃。 结果： 杨梅苷，异槲皮苷和山柰酚-4'-O-β-D-吡喃葡萄糖苷进样量分别在0.048~0.482 μg（r=0.9999）、0.023~0.228 μg（r=0.9997）和0.040~0.404 μg（r=0.9999）范围内与峰面积呈良好线性关系；平均回收率（n=6）分别为99.9%、98.9%和99.4%，RSD分别为1.3%、1.4%和1.1%。 结论： 经方法学验证，本法可用于枫香树叶药材的质量控制。

Objective: To develop an HPLC method for the simultaneous determination of the contents of myricitrin,isoquercitrin and kaempferol-4'-O-β-D-glucopyranside in the leaves of Liquidambar formosana Hance. Methods: The separation was performed on a YMC-Pack ODS-A column(150 mm×4.6 mm,5 μm) eluted with a mobile phase of acetonitrile-0.2% phosphoric acid aqueous solution(13: 87) at a flow rate of 1.0 mL·min^-1,and the detection wavelength was 351 nm and the column temperature was 25 ℃. Results: The linear ranges of myricitrin,isoquercitrin and kaempferol-4'-O-β-D-glucopyranside were 0.048-0.482 μg(r=0.9999),0.023-0.228 μg(r=0.9997) and 0.040-0.404 μg(r=0.9999),respectively.The average recoveries(n=6) were 99.9%(RSD=1.3%),98.9%(RSD=1.4%) and 99.4%(RSD=1.1%),respectively. Conclusion: The method was validated.It could be used for quality control of the leaves of Liquidambar formosana Hance.