HPLC法测定香薷散不同煎液中香荆芥酚和麝香草酚的含量及体外抗菌作用比较

目的： 考察用合煎、分煎2种方法制备的香薷散中香荆芥酚和麝香草酚含量的变化并比较其体外抗菌作用。 方法： （1）有效成分含量测定用HPLC法，采用Phenomenex Luna C₁₈色谱柱（4.6 mm×250 mm，5 μm），以甲醇-5%乙酸水（60：40）为流动相，流速1.0 mL·min^-1，检测波长274nm，柱温30 ℃；（2）采用微量稀释法体外测试香薷散不同煎液对常见菌种的最低抑菌浓度。 结果： （1）合煎液中香荆芥酚和麝香草酚的平均含量高于分煎液；（2）合煎液对金黄色葡萄球菌、乙型溶血性链球菌和绿脓杆菌的抑菌作用大于分煎液。 结论： 与分煎液比较，合煎液中香荆芥酚和麝香草酚的含量较高，抑菌作用较强。

Objective: To investigate the contents of carvacrol and thymol in the combined decoction and the separated decoction of Xiangrusan (composed of Mosla chinensis Maxim.,Magnolia officinalis Rehd.et Wils.and Dolichos lablab L.) and to compare their anti-bacterial effects in vitro. Methods: (1)The samples were separated on a Phenomenex Luna C₁₈ column(4.6 mm×250 mm,5 μm) with methanol-water containing 5% acetic acid(60: 40) at a flow rate of 1 mL·min^-1.The detection wavelength was 274 nm and the column temperature was controlled at 30 ℃.(2)Minimal bacterial inhibitory concentrations (MIC) against the common bacteria in different decoctions were measured in vitro by the micro dilution method. Results: (1)The average contents of carvacrol and thymol in the combined decoction were higher than those in the separated decoction.(2)The antibacterial effect of the combined decoction against Staphylococcus aureus,β-Hemolytic streptococcus and Pseudomonas aeruginosa was stronger than that of the separated decoction. Conclusion: Compared with the separated decoction,the contents of carvacrol and thymol in the combined decoction were higher and the anti- bacterial effect was stronger.

[1] XU Ji-qun(许济群),WANG Mian-zhi(王绵之).Formulas of Traditional Chinese Medicine(方剂学)[M].Beijing(北京):People's Medical Publishing House(人民卫生出版社),2008:179
[2] GE Bing(葛冰),LU Xiang-yang(卢向阳),JIANG Hong-mei(蒋红梅),et al.Study on the anti-bacterial effects in vitro of Mosla chinensis Maxim.volatile oil (石香薷挥发油体外抗菌作用研究)[J].Chin J Tradit Vet Sci(中兽医学杂志),2005(2):8
[3] CHENG Cai-lian(成彩莲),PENG Cheng-xiu(彭承秀),LIU Ai-rong(刘爱荣).Observation on the anti-bacterial effects of volatile oil of Mosla chinensis Maxim.and curative effect in the treatment of acute bacillary dysentery(石香薷挥发油抗菌作用及治疗急性细菌性痢疾的疗效观察) [J].J Tongji Med Univ(同济医科大学学报),2000,29(6) :569
[4] DING Chen-xu(丁晨旭),JI Lan-ju(纪兰菊).Research advance of the chemical component and pharmacological action of elsholtzia(香薷化学成分及药理作用研究进展) [J].Shanghai J Tradit Chin Med(上海中医药杂志),2005,39(5):63
[5] WANG Fang-yin(王放银),DUAN Lin-dong(段林东).Comparative study on the antibacterial effect of Mosla chinensis Maxim.volatile oil(石香薷挥发油抗菌效果的比较研究) [J].Feed Ind Mag(饲料工业),2004,25(10):31
[6] ZHANG Jun-tian(张均田).Method of Modern Pharmacology Experiment(现代药理实验方法) [M].Beijing(北京):Beijing Medicine University Peking Union Medical College United Publishing House(北京医科大学中国协和医科大学联合出版社),1998:1412
[7] HE Jia(何佳),CHEN Jun(陈钧),ZHAO Qi-mei(赵启美),et al. Study on screening antimicrobial activity of endophytes fungus from Pseudolarix kaempferi Gord with microdilution method(微量稀释法筛选金钱松内生真菌抗菌活性的研究) [J].Food Sci(食品科学),2007,28(4):199
[8] CAI Qing(蔡晴),LI Shan-shan(李珊山),SONG Yang(宋洋),et al. In vitro antifungal susceptibility of yeast phase of by Sporothrix schenckii by microdilution broth method(微量稀释法检测申克孢子丝菌酵母相体外抗真菌药物敏感性) [J].Chin J Dermatovenereol(中国皮肤性病学杂志),2011,25(5):355
[9] DENG Zhong-jia(邓中甲).Formulas of Traditional Chinese Medicine(方剂学)[M].Beijing(北京):China Press of Traditional Chinese Medicine(中国中医药出版社),2010:190
[10] JI Chang-xin(姬长新),JIAO Lei(焦镭),ZHU Wei-jun(朱维军),et al. Study on anti-bacterial effects(厚朴的抗菌作用研究) [J].Agric Henan(河南农业),2010(8):50
[11] LI Xing-cai(李星彩).Chemical composition and antimicrobial activity of the essential oil from the leaf of Magnolia officinalis(厚朴叶挥发油化学成分分析及其抗菌活性研究) [J].Food Sci Technol(食品科技),2013,38(1):271
[12] ZHANG Shu-jie(张淑洁),ZHONG Ling-yun(钟凌云).The research progress of Magnolia officinalis chemical components and modern pharmacology(厚朴化学成分及其现代药理研究进展) [J].J Chin Med Mater(中药材),2013,36(5):838