一测多评法同时测定川芎、当归饮片中多种化学成分的含量

目的：通过建立川芎、当归中阿魏酸、洋川芎内酯Ⅰ、洋川芎内酯A、藁本内酯的一测多评含量测定方法，同时实现川芎、当归饮片的多指标控制。方法：采用Agilent ZORBAX SB-C₁₈（4.6 mm×150 mm，3.5 μm）色谱柱，以乙腈-1%甲酸水溶液为流动相，梯度洗脱（0~10 min，10%A→28%A；10~17 min，28%A→45%A；17~25 min，45%A→65%A；25~30 min，65%A→100%A；30~35 min，100%A；35~37 min，100%A→10%A；37~45 min，10%A），流速0.8 mL·min^-1，检测波长280 nm，柱温25 ℃，以阿魏酸为内标，建立洋川芎内酯Ⅰ、洋川芎内酯A、藁本内酯的相对校正因子，利用相对校正因子计算4个成分的含量，实现一测多评；同时采用外标法测定这4个成分的含量，将2种方法进行分析比较。结果：2种方法测定结果没有明显差异。结论：同时建立了川芎饮片、当归饮片中阿魏酸、洋川芎内酯Ⅰ、洋川芎内酯A、藁本内酯4个成分一测多评法，经方法学验证，该法可用于川芎、当归的质量控制。

Objective: To develop a quantitative analysis of multi-components by single maker(QAMS) method for simultaneous determination of four components (ferulic acid,senkyunolide Ⅰ,senkyunolide A,ligustilide) in decoction pieces of Chuanxiong Rhizoma and Angelicae Sinensis Radix and achieve multi-index control. Methods: The Agilent ZORBAX SB column (4.6 mm×150 mm,3.5 μm) was adopted;the mobile phase consisted of acetonitrile-1% formic acid water with gradient elution (0-10 min,10%A→28%A;10-17 min,28%A→45%A;17-25 min,45%A→65%A;25-30 min,65%A→100%A;30-35 min,100%A;35-37 min,100%A→10%A;37-45 min,10%A) at a flow of 0.8 mL·min^-1;the detection wavelength was 280 nm,and the column temperature was 25 ℃.Ferulic acid was used as the internal reference standard,and 3 relative correction factors (RCF) to ferulic acid were calculated.Their content in decoction pieces of Chuanxiong Rhizoma and Angelicae Sinensis Radix was determined by both external standard method and QAMS. Results: There were no significant differences between the results of the two methods. Conclusion: The method of QAMS for four components in decoction pieces of Chuanxiong Rhizoma and Angelicae Sinensis Radix was established.The methodology validation showed that the method can be used for quality control of decoction pieces of Chuanxiong Rhizoma and Angelicae Sinensis Radix.