HPLC法同时测定濒危植物——中华补血草中异槲皮苷、桑色素、槲皮素、木犀草素和芹菜素的含量

目的： 建立HPLC法测定濒危野生药用植物中华补血草（Limonium sinense （Girard） Kuntze） 中异槲皮苷、桑色素、槲皮素、木犀草素和芹菜素的含量，为中华补血草的质量控制提供理论依据。 方法： 采用高效液相色谱法同时测定中华补血草中异槲皮苷、桑色素、槲皮素、木犀草素和芹菜素的含量。色谱柱：TURNER Kromasil^TM C₁₈柱（250 mm×4.6 mm，5 μm），以甲醇-0.4%磷酸溶液为流动相进行梯度洗脱，流速为1 mL·min^-1，检测波长为360 nm，柱温30℃。 结果： 异槲皮苷、桑色素、槲皮素、木犀草素和芹菜素的质量浓度与峰面积分别在1.00～99.67 mg·L^-1（r=0.9994）、0.45～45.42 mg·L^-1（r=0.9997）、0.77～76.57 mg·L^-1（r =0.9996）、0.43～42.72 mg·L^-1（r =0.9997）、0.55～55.39 mg·L^-1 （r =0.9998） 范围内呈良好的线性关系；平均加样回收率（n=6）分别为98.9%、98.6%、98.6%、97.8%、98.7%，RSD为1.6%、1.7%、1.7%、2.2%、1.7%。 结论： 方法简便可行、重复性好，适合于同时测定中华补血草中异槲皮苷、桑色素、槲皮素、木犀草素和芹菜素的含量，可作为我国中华补血草质量评价的方法之一。

Objective: To establish a determination method for the content of isoquercetin,morin,quercetin,luteolin and apigenin in Limonium sinense (Girard) Kuntze by HPLC and provide theoretical support for quality control of this medicine. Methods: The HPLC conditions with TURNER Kromasil^TMC₁₈ column (250 mm×4.6 mm,5 μm) were used;the mobile phase consisted of methanol-0.4% phosphoric acid;the flow rate was 1 mL·min^-1 and the detection wavelength was set at 360 nm.The column temperature was 30℃. Results: The linear ranges for isoquercetin,morin,quercetin,luteolin,apigenin were 1.00-99.67 mg·L^-1 (r=0.9994),0.45-45.42 mg·L^-1 (r=0.9997),0.77-76.57 mg·L^-1 (r=0.9996),0.43-42.72 mg·L^-1 (r =0.9997),0.55-55.39 mg·L^-1 (r=0.9998),respectively.The average recoveries(n=6) were 98.9%,98.6%,98.6%,97.8% and 98.7% with RSDs of 1.6%,1.7%,1.7%,2.2%,1.7%,respectively. Conclusion: The method is simple and repeatable.This method could be used to simultaneously determinate the contents of isoquercetin,morin,quercetin,luteolin and apigenin in Limonium sinense (Girard) Kuntze,and to identify and evaluate the quality of Limonium sinense (Girard) Kuntze.