HPLC法同时测定益肝康颗粒中没食子酸、丹参素、芍药苷、丹酚酸B和丹参酮Ⅱ_A含量

目的：采用HPLC 法同时测定益肝康颗粒（丹参、黄芪、赤芍、当归等）中活性成分没食子酸、丹参素、芍药苷、丹酚酸B和丹参酮Ⅱ_A的含量。方法：采用C₁₈反相色谱柱，以甲醇-乙腈-0.1% 磷酸水溶液为流动相梯度洗脱，检测波长为芍药苷230 nm，没食子酸、丹参素、丹酚酸B、丹参酮Ⅱ_A 270 nm 。结果：没食子酸、丹参素、芍药苷、丹酚酸B和丹参酮Ⅱ_A进样量分别在30.1~376.8、452.7~5659.2、201.6~2520、1657.0~20712.0、54.336~679.2 μg·mL^-1范围内呈良好的线性关系，平均回收率分别为102.5%、104.7%、99.4%、105.0%、102.9%。结论：该方法简捷，快速，可靠，可用于控制该制剂的质量。

Objective: To develop a method for determining the contents of gallic acid,tanshinol,paeoniflorin,salvianolic acid B and tanshinone Ⅱ_A in Yi gan kang granules. Methods: The analytical column was an ODS column.The mobile phase consisted of acetonitrile-0.1% phosphoric acid aqueous solution using gradient elution.The detection wavelength of paeoniflorin was 230 nm,and the wavelength for gallic acid,tanshinol,salvianolic acid B and tanshinone Ⅱ_A detection was set at 270 nm. Results: The calibration curves of gallic acid,tanshinol,paeoniflorin,salvianolic acid B and tanshinone Ⅱ_A showed good linearity in the ranges of 30.1-376.8, 452.7-5659.2, 201.6-2520,1657.0-20712.0 and 54.336-679.2 μg·mL^-1,and the average recoveries were 102.5%,104.7%,99.4%,105.0% and 102.9%,respectively. Conclusions: The method is simple,rapid,reliable and can be used for drug quality control.This method replenishes the insufficient quality control of Yi gan kang granules.

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