拉曼光谱法测定氯膦酸二钠注射液的含量
Quantitative determination of clodronate disodium injection by Raman spectroscopy
单位(英文)：1. China Pharmaceutical University, Nanjing 210009, China;
2. Yancheng Institute for Drug Control, Yancheng 224002, China;
3. Jiangxi University of Traditional Chinese Medicine, Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Nanchang 330004, China;
4. Jiangsu Institute for Food and Drug Control, Nanjing 210008, China
分类号：
出版年·卷·期（页码）：2014,34 (2):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的：建立拉曼光谱法测定氯膦酸二钠注射液的含量的方法。方法：利用拉曼散射原理，在水溶液中，拉曼峰信号的强度与溶质分子的浓度成正比，选取样品溶液拉曼光谱中氯膦酸二钠的P=O基团振动（1079 cm-1）处的强峰作为定量峰，建立拉曼光谱法测定氯膦酸二钠注射液含量的方法。结果：氯膦酸二钠在2~100 mg·mL-1范围内与拉曼峰强度呈良好的线性关系，线性回归方程为Y=426.0X+286.6，相关系数为0.9999（n=8），平均回收率为102.8%，RSD为1.6%（n=9）。结论：本方法操作简便、快速，可用于氯膦酸二钠注射液的含量测定。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To establish a Raman method for the determination of clodronate disodium injection. Methods: Based on the Raman scattering theory that the light intensity of the laser Raman peak is in proportional to the concentration of clodronate disodium samples in aqueous solution, a quantitative method for determination of clodronate disodium injection using Raman spectroscopy was established by selecting the Raman peak of clodronate disodium P=O bond (P=O[1079 cm-1]) as the quantitative peak. Results: The calibration curve was linear over the range of 2-100 mg·mL-1 (r= 0.9999,n=8),and the average recovery was 102.8% with RSD of 1.6% (n=9). Conclusion: The method is convenient and rapid,which can be used for the quality control of clodronate disodium injection.
-----参考文献：---------------------------------------------------------------------------------------
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