液-质联用法测定妇宁丸中盐酸水苏碱的含量

目的：建立妇宁丸中盐酸水苏碱的含量测定方法。方法：采用LC-MS/MS法测定盐酸水苏碱的含量。使用Venusil HILIC色谱柱（100 mm×2.1 mm，3 μm），以乙腈-水（75：25）为流动相，流速0.3 mL·min^-1；以电喷雾离子源（ESI）正离子模式将样品离子化，多反应监测（MRM）模式下对盐酸水苏碱（m/z 144.0→57.91和m/z 144.0→83.96）进行测定。结果：盐酸水苏碱浓度在5.0440~201.76 ng·mL^-1线性关系良好（r=0.9999），平均回收率（n=6）为99.6%，RSD为1.3%。结论：该方法简便、可靠、准确，可作为妇宁丸中盐酸水苏碱的含量测定方法。

Objective: To establish the quantitative method of determining the contents of stachydrine hydrochloride in Funing pills. Methods: The content of stachydrine hydrochloride in Funing pills was determined by LC-MS/MS.The chromatographic column was Venusil HILIC(100 mm×2.1 mm,3 μm).The mobile phase was acetonitrile-water (75:25),at the flow rate of 0.3 mL·min^-1.The eletrospray ionization(ESI) with positive source of the mass spectrometer and detected in multiple reaction monitor(MRM) mode was used to detect stachydrine hydrochloride(m/z 144.0→57.91 and m/z 144.0→83.96). Results: The linear range of stachydrine hydrochloride was 5.0-201.8 ng·mL^-1(r=0.9999).The average recovery rate(n=6) was 99.6%,and RSD was 1.3%. Conclusion: This method is simple,accurate and can be used to determine the content of stachydrine hydrochloride in Funing pills.

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