高效液相色谱法测定甜叶菊中的3个甜菊醇糖苷的含量

目的：建立高效液相色谱法分析甜叶菊中3个甜菊醇糖苷含量。方法：采用Poroshell 120 EC-C₁₈色谱柱（4.6 mm×150 mm，2.7 μm），以乙腈-磷酸水溶液（30：70）为流动相进行分离，在紫外检测波长210 nm下进行检测，外标法定量。结果：3个甜菊醇糖苷的检出限为1.0～1.2 mg·kg^-1，定量限为2.5～3.0 mg·kg^-1;在50～1000 mg·L^-1的浓度范围内线性良好，相关系数为0.9999～1.000;瑞鲍迪苷A、瑞鲍迪苷C和甜菊苷的加标回收率分别为92.8%、87.2%、90.3%，RSD分别为3.9%、2.7%、4.8%;精密度的RSD分别为1.2%、2.1%、1.3%；重复性的RSD分别为3.3%、6.4%和4.7%。结论：本方法操作简便，快速，分离度和准确度高，可用于甜叶菊原料的质量控制。

Objective: To develop a method for the determination of rebaudioside A,rebaudioside C and stevioside in Stevia rebaudiana Bertohi by HPLC. Methods: The analytes were separated on a Poroshell 120 EC C₁₈ column(4.6 mm×150 mm,2.7 μm),eluted by 30%acetonitrile and 70% phosphoric acid water solution,identified by UV detector at 210 nm and quantified by external standard method. Results: The limits of detection(LOD,S/N=3) and quantification (LOQ,S/N=10) were 1.0-1.2 mg·kg^-1 and 2.5-3.0 mg·kg^-1 respectively. The correlation coefficients of linear calibration curves were 0.9999-1.000 in the range from 50-1000 mg·L^-1. The recoveries of rebaudioside A,rebaudioside C and stevioside were 92.8%,87.2% and 90.3% and the RSDs were 3.9%,2.7% and 4.8%. The precision RSDs were 1.2%,2.1%,1.3% respectively,and the repeatability RSDs were 3.3%,6.4% and 4.7% respectively. Conclusion: The experiment results indicate that the method is simple and rapid with good separation and accuracy,and can be used for the quality control of the raw material of Stevia rebaudiana Bertohi.

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