土牛膝及广东土牛膝的性状、显微比较研究

目的: 对市场上出现的土牛膝混淆品进行鉴定，并建立土牛膝及其混淆品的性状及显微鉴别方法。方法: 在植物分类学鉴定的基础上，对土牛膝的混淆品广东土牛膝进行鉴定，并使用性状与显微的研究方法，对采集及市场上收集土牛膝及广东土牛膝样品进行比较分析。结果: 通过研究发现，土牛膝与广东土牛膝可以通过性状、根横切面及粉末的显微特征进行区分，两者区别明显。结论: 土牛膝与广东土牛膝可以通过性状、显微等方面进行鉴别；通过对两者性状、显微的细致研究，为相关标准的修订及提高提供依据。

Objective: To identify adulterant species of Radix et Rhizoma Achyranthis Asperae found in the market and establish methods for morphological and histological identification of Radix et Rhizoma Achyranthis Asperae and its adulterant species. Methods: Based on plant taxonomy,the adulterant species of Radix et Rhizoma Achyranthis Asperae was identified;based on morphological and microscopic research,samples of Radix et Rhizoma Achyranthis Asperae and Radix Eupatorii Chinensis collected from the field and the market were compared. Results: The morphological and microscopic differences between Radix et Rhizoma Achyranthis Asperae and Radix Eupatorii Chinensis were obvious and could be distinguished according to their properties,transverse section of root and microscopic characteristics.Conclusion: Radix et Rhizoma Achyranthis Asperae and Radix Eupatorii Chinensis can be identified by comparing their morphology and microscopic structures,and the results provide evidence for revision and perfection of related standards.

1 ChP(中国药典)[S].2010.Vol I(一部):Appendix(附录)22
2 Hunan Food and Drug Administration(湖南省食品药品监督管理局).Chinese Medicinal Materials Standard of Hunan Province(湖南省中药材标准)[M].Changsha(长沙):Hunan Science and Technology Press(湖南科学技术出版社),2009.292
3 HUANG Zhi-fang(黄志芳),HUANG Zhi-hong(黄志红),HU Ji-lin(胡吉林), et al.Extracting and identification of OA and ABPS of local Achyranthes aspera(土牛膝中齐墩果酸、牛膝多糖的提取与鉴定) [J].China Pract Med(中国实用医药),2010,5(17):6
4 Subbarayan PR,Sarkar M,Impellizzeri S,et al.Anti-proliferative and anti-cancer properties of Achyranthes aspera:Specific inhibitory activity against pancreatic cancer cells[J].J Ethnopharmacol,2010,131(1):78
5 LI Wei-ping(李伟平),HE Liang-yan(何良艳),MA Zhe-long(马哲龙),et al.Anti-inflammatory and analgesic effects of polysaccharides from Radix Achyranthis Sylvestris(土牛膝多糖抗炎镇痛作用的研究) [J].Chin Arch Tradit Chin Med(中华中医药学刊),2012,30(4):747
6 Guangdong Food and Drug Administration(广东省食品药品监督管理局).Chinese Medicinal Materials Standard of Guangdong Province(广东省中药材标准)[M].Vol 1(第一册).Guangzhou(广州):Guangdong Science and Technology Press(广东科技出版社),2004.21
7 ZHANG Dan-yan(张丹雁),WANG Hong(王宏),LAI Xiu-zhen(赖秀珍).Preliminary study of chemical component in Radix Eupatorii Chinensis(广东土牛膝化学成分的初步研究) [J].Chin J Ethnomed Ethnopharm(中国民族医药杂志),1999,39:232
8 LIU Xiao-yan(刘晓燕),ZENG Xiao-chun(曾晓春),JIANG Jian-dong(江剑东).Anti-inflammatory and analgesic effects research of Radix Eupatorii Chinensis(广东土牛膝抗炎镇痛的研究) [J].Chin Arch Tradit Chin Med(中医药学刊),2004,22(8):1566
9 YING Jun(应军) ,LUO Xiao-ping(罗小萍).Morphological and microscopic identification of Radix Eupatorii Chinensis(广东土牛膝的性状与显微鉴别) [J].J Chin Med Mater(中药材),2004,27(9):635
10 CAI Xiao-qun(蔡晓群),LI Shu-yuan(李书渊).Pharmacognosy research of Eupatorii Chinensis Radix(广东土牛膝的生药学研究) [J].J Sichuan Tradit Chin Med(四川中医),2012,30(11):52
11 ZHANG De-pei(张德培),HUANG Hai-bo(黄海波),ZHENG Li-dan(郑丽丹).The identification of Radix Eupatorii Chinensis and Radix et Rhizoma Achyranthis Asperae(广东土牛膝与土牛膝的鉴别方法) [J].China Health Ind(中国卫生产业),2012,10:99
12 HU Zheng-hai(胡正海),ZHANG Hong(张泓).Plant Anomalous Structure Anatomy( 植物异常结构解剖学) [M].Beijing(北京):Higher Education Press(高等教育出版社),1993.62