不同加工方法对泸州山银花品质影响的研究

目的: 探讨不同加工方法对泸州山银花品质的影响，为泸州山银花加工方法的筛选提供依据。方法: 采用阴干、晒干、真空干燥、远红外干燥、微波干燥、烘干6种方法加工山银花，对外观性状进行描述，采用高效液相色谱法测定山银花中新绿原酸、绿原酸、异绿原酸A、异绿原酸C的含量。色谱柱为Kromasil C₁₈柱(250 mm×4.6 mm，5 μm)；流动相为乙腈-0.1%磷酸水溶液，梯度洗脱；检测波长326 nm；柱温30℃；流速1 mL·min^-1；进样量10 μL。结果: 6种加工方法中，真空干燥样品中新绿原酸、绿原酸、异绿原酸A及异绿原酸C的含量分别为0.094%、2.982%、1.804%、0.255%，高于其他5种方法所得样品；外观颜色和油润度均比其他5种方法好。结论: 真空干燥方法得到的样品外观品相好，测得的新绿原酸、绿原酸、异绿原酸A及异绿原酸C含量高，明显优于其他干燥方法。

Objective: To discuss the effects of different drying methods on officinal qualities of Flos Lonicerae. Methods: The following six methods were used:drying in the shade,drying in the sun,vacuum drying,far infrared drying,microwave drying,and oven drying.External qualities were studied and the contents of neochlorogenic acid,chlorogenic acid,isochlorogenic acid A and isochlorogenic acid C were determined by HPLC.HPLC was performed on a Kromasile C₁₈ column(250 mm×4.6 mm,5 μm)at 30℃.The composition of mobile phase was acetonitrile-0.1% phosphoric acid with gradient elution,the flow rate was 1 mL·min^-1,and the injection volume was 10 μL. Results: The content of neochlorogenic acid,chlorogenic acid,isochlorogenic acid A and isochlorogenic acid C in the vacuum drying sample was 0.094%,2.982%,1.804% and 0.255% respectively,which was obviously higher than samples dried by other five methods;the color and texture of the vacuum drying sample was also better.Conclusion: The appearance and contents of chlorogenic acids of the vacuum drying sample are better than those of the others,indicating that the vacuum drying method is obviously better than the others.