目的: 采用一测多评，建立槐角中异黄酮类成分的含量测定方法。方法: 采用Agilent Extend C18色谱柱 (250 mm × 4.6 mm，5 μm)，以甲醇-乙腈-0.1%磷酸水溶液为流动相，流速为1.0 mL·min-1，柱温为30℃，检测波长为260 nm，建立槐角苷与槐属双苷、染料木苷和染料木素的相对校正因子，并以相对保留时间作为目标峰的定位标准。结果: 色谱峰分离度良好，槐角苷在0.374~7.49 μg、槐属双苷在2.16 × 10-2~0.432 μg、染料木苷在2.61 × 10-2~0.522 μg、染料木素在1.02×10-2~0.203 μg呈良好线性关系，平均加样回收率槐角苷为100.7%、槐属双苷为96.3%、染料木苷为98.6%、染料木素为100.8%。槐角苷对槐属双苷、染料木苷和染料木素的相对校正因子分别为0.80、1.03、1.61。相对保留时间分别为0.42、0.81、1.26。结论: 采用一测多评法测定槐角中异黄酮类成分的含量是准确、可行的。
Objective: To establish a method for the determination of the isoflavone content in Fructus Sophorae by quantitative analysis of multi-components by single marker (QAMS). Methods: The relative correction factors (RCFs) of sophorabioside,genistin and genistein to that of sophoricoside were calculated on an Agilent Extend C18 column (250 mm×4.6 mm,5 μm) with methanol-acetonitrile-0.1% phosphoric acid aqueous solution as the mobile phase.The flow rate was 1.0 mL·min-1,the column temperature was set at 30℃,and the detector wavelength was 260 nm.The relative retention time (RRT) was used to define the target peak. Results: Sophoricoside,sophorabioside,genistin,and genistein showed a good linear relationship within ranges of 0.374-7.49 μg,2.16×10-2-0.432 μg,2.61×10-2-0.522 μg,and 1.02×10-2-0.203 μg,respectively,and the recoveries were 100.7%,96.3%,98.6%,and 100.8%,respectively.The RCFs of sophorabioside,genistin and genistein were 0.80,1.03,1.61,and the RRTs were 0.42,0.81,1.26,respectively.Conclusion: The QAMS method is simple,rapid,accurate and reliable,and it can be applied to determine the content of isoflavones in Fructus Sophorae.
