UPLC法同时测定酒花提取物中4个黄酮成分的含量

目的: 建立同时测定酒花提取物中芸香苷、紫云英苷、槲皮素和黄腐酚4个黄酮成分含量的UPLC法。 方法: 采用Hypersil BDS C₁₈色谱柱(4.6 mm×250 mm,5 μm),以乙腈-1%甲酸水溶液为流动相,梯度洗脱,体积流量0.8 mL·min^-1,柱温25℃, 检测波长为350 nm。 结果: 芸香苷、紫云英苷、 槲皮素和黄腐酚分别在 0.011~0.187 μg、0.003~0.054 μg、0.011~0.194 μg和0.016~0.265 μg范围内线性关系良好,相关系数(r)分别为 0.9978、0.9968、0.9998和0.9999,平均加样回收率分别为99.5 %、103.7 %、101.6 %和101.7 %,RSD分别为2.2 %、1.5%、2.2 %和2.4 %。酒花黄酮提取物中芸香苷、紫云英苷、槲皮素和黄腐酚的含量分别为4.57、0.60、1.03和13.28 mg·g^-1。 结论: 该方法简便快速,准确可靠,重复性好,可用于酒花药材和提取物的质量评价。

Objective: To establish a UPLC method for simultaneous determination of four components in flavonoid extract of H.lupulus including rutin,astragalin,quercetin and xanthohumol. Methods: The analysis was performed on a Hypersil BDS C₁₈(4.6 mm×250 mm,5 μm)with the mobile phase consisting of acetonitrile and 1% formic acid aqueous at a flow rate of 0.8 mL·min^-1.The detection wavelength was 350 nm,and column temperature was 25℃. Results: The standard curve revealed a good linear relationship over the range of 0.011-0.187 μg for rutin,0.003-0.054 μg for astragalin,0.011-0.194 μg for quercetin,0.016-0.265 μg for xanthohumol.Their average recovery rates were 99.5%(RSD=2.2%),103.7%(RSD=1.5%),101.6%(RSD=2.2%),and 101.7%(RSD=2.4%),respectively.The contents of rutin,astragalin,quercetin,and xanthohumol in the flavonoid extract were 4.57,0.60,1.03 and 13.28 mg·g^-1,respectively. Conclusion: The established determination method is simple,rapid,accurate,sensitive,reliable and reproducible,which can be used for qualitative evaluation of H.lupulus and its extract.