人乳头瘤病毒全基因组基因分型国家参考品的建立

目的: 建立包含20种不同型别和5个阴性参考品的人乳头瘤病毒全基因组基因分型国家参考品,为人乳头瘤病毒基因分型检测、对试剂盒进行质控和分型检测试剂盒评价提供质控物。 方法: 通过对本科室保存的人乳头瘤病毒全基因组质粒进行提取、测序,用测序正确的质粒连同收集的阴性参考品组成人乳头瘤病毒全基因组基因分型参考品盘,并用不同的方法对本套参考品进行验证。 结果: 采用不同的验证方法对本参考品进行验证,结果表明,参考品中20个型别的阳性参考品其型别与验证结果一致,5个阴性参考品验证结果均为阴性。 结论: 成功建立了包含20种不同型别和5个阴性参考品的人乳头瘤病毒全基因组基因分型国家参考品。

Objective: To establish the national reference for genotyping of human papillomavirus (HPV) genome including 20 different subtypes and 5 negative references and to provide quality control materials for the quality control of genotyping detection and evaluation of genotyping detection kits. Methods: The stored genome plasmid of human papillomavirus in our laboratory was extracted and sequenced,the reference panel was produced using the collected negative reference materials and the correct plasmid,then the reference panels were verified by various methods. Results: The verification of the reference by various verification methods demonstrated that the 20 types of positive references were consistent with the result and all the 5 negative references were conformed to be negative. Conclusion: The national references for genotyping of human papillomavirus genome including 20 genotypes and 5 negative references were established successfully.

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