UFLC法同时测定新型香料中2个毒品成分的含量

目的: 建立超快速液相色谱法同时测定新型香料中1-丁基-3-(1-萘甲酰基)吲哚(JWH-073)和1-戊基-3-(1-萘甲酰基)吲哚(JWH-018)的含量。 方法: 采用超快速液相色谱法。色谱柱: Shim-pack XR-ODS Ⅱ柱(2.0 mm×75 mm,2.2 μm);流动相: 乙腈-水,梯度洗脱(0~10 min, 65%→85%乙腈;10~11 min,85%→65%乙腈;11~15 min,65%乙腈);流速: 0.25 mL·min^-1;检测波长: 280 nm,柱温: 35 ℃,进样量: 5 μL。外标法定量测定JWH-073与JWH-018这2个组分的含量。 结果: JWH-073与JWH-018的线性关系良好,平均回收率(n=9)分别为98.58%、98.72%。 结论: 本法线性良好,灵敏度高,重复性好,简便可行,可用于同时测定新型香料中JWH-073与JWH-018的含量。

Objective: To establish an UFLC method for simultaneous determination of two compositions(JWH-073, JWH-018) in a new type spice. Methods: A UFLC method was applied with a Shim-pack XR-ODS Ⅱ chromatographic column (2.0 mm×75 mm, 2.2 μm), the mobile phase consisted of acetonitrile and water, eluted in gradient mode(0-10 min, 65%→85% acetonitrile; 10-11 min, 85%→65% acetonitrile; 11-15 min, 65% acetonitrile). The mobile phase was pumped at a flow rate of 0.25 mL·min^-1. The UV detection wavelength was set at 280 nm. The column temperature was controlled at 35 ℃. The injection volume was 5 μL. The contents of JWH-073 and JWH-018 in the new type spice were calculated by external standardization method. Results: There were good linear relationships for JWH-073 and JWH-018. The average recoveries(n=9) were 98.58%, 98.72%, respectively. Conclusion: The established UFLC method is simple, sensitive, reproducible and feasible for simultaneous determination of the two compositions, JWH-073 and JWH-018 in the new type spice.

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