柱切换离子色谱法测定厄贝沙坦中的叠氮根离子

目的:用双泵柱切换离子色谱系统直接进样测定厄贝沙坦中的痕量叠氮根离子。方法: 采用Dionex IonPac NG1柱将所需分析的痕量N₃^-从厄贝沙坦基质中分离出来,再将被分析组分通过Dionex TAC-ULP1进行浓缩,最后将浓缩的N₃^-通过Dionex IonPac AS18+AG18分析柱进行分离,抑制性电导检测器进行检测。结果: 方法重复性良好,回收率在95.4%~98.9%之间,最低检出限为3 ng·mL^-1,在0.04~0.40 μg·mL^-1浓度范围内线性关系良好。结论: 本文方法可以在脂溶性药物的痕量离子测定中广泛应用。

Objective: To establish a method for the simultaneous determination of azide ion(N₃^-)in irbesartan with double pump and column-switching ion chromatography. Methods: An ion excluding column Dionex IonPac NG1 was used for the separation of azide ion from irbesartan.A pre-concentration column Dionex TAC-ULP1 was used to enrich the trace azide ion.After being switched to analytical system,the separation was performed on Dionex ICS3000 using Dionex IonPac AG18 as guard column,Dionex IonPac AS18 as analytical column,Dionex ASRS4mm as suppressor conductivity detector. Results: The RSDs of retention time and peak area were good.The recoveries of azide ion were between 95.4%-98.9%.Analyte calibration curves of analytes were linear in the range between 0.04 μg·mL^-1 and 0.40 μg·mL^-1.Limit of detection of the method was 3 ng·mL^-1. Conclusion: This method can be applied to the detection of the trace anions in fat-soluble drug.

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