HPLC法测定复方两面针含片中苦参碱和没食子酸的含量

目的: 建立复方两面针含片中苦参碱和没食子酸的HPLC含量测定方法。 方法: 色谱柱为ZORBAX Eclipse XDB C₁₈(250 mm×4.6 mm,5 μm)柱;流动相为乙腈-甲醇-磷酸盐缓冲液(pH 6.8)-三乙胺(16:16:68:0.1)和乙腈-0.1%磷酸0.1%三乙胺水溶液(3:97),流速1.0 mL·min^-1;柱温30℃;检测波长220 nm。 结果: 苦参碱在0.10~2.00 μg范围内线性关系良好(r=0.99960,n=6);平均回收率为99.4%(n=9)。没食子酸在0.011~1.75 μg范围内线性关系良好(r=0.99998, n=6);平均回收率为99.1%(n=9)。 结论: 建立的方法适用于复方两面针含片的质量控制。

Objective: To establish an analytical method for determination of matrine and gallic acid in Compound Liangmianzhen tablets by HPLC. Methods: The analysis was achieved on a ZORBAX Eclipse XDB C₁₈ column(250 mm×4.6 mm,5 μm)using a mobile phase of acetonitrile-methanol-phosphate buffer(pH 6.8)-triethylamine(16:16:68:0.1)and a mobile phase of acetonitrile-0.1% phosphoric acid and 0.1% triethylamine water solution(3:97)as the mobile phase;the flow rate was 1.0 mL·min^-1;the detecting wavelength was 220 nm and the column temperature was 30℃. Results: The calibration curve of matrine was linear within the range of 0.10-2.00 μg(r=0.9996,n=6),the average recovery was 99.4%(n=9).The calibration curve of gallic acid was linear within the range of 0.011-1.75 μg(r=0.9999,n=6),the average recovery was 99.1%(n=9). Conclusion: The method can be used to control the quality of the Compound Liangmianzhen tablets.

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