HPLC-ELSD法同时测定盾叶薯蓣根茎中5个皂苷的含量

目的: 建立同时测定盾叶薯蓣根茎中黄姜素A、盾叶新苷、三角叶薯蓣皂苷、薯蓣皂苷及纤细皂苷5个成分含量的HPLC-ELSD分析方法,并测定不同产地盾叶薯蓣根茎中上述5个成分的含量。 方法: 采用Welchrom C₁₈(250 mm×4.6mm,5 μm)色谱柱;流动相:乙腈(A)-水(B),梯度洗脱(0~5 min,25%A→30%A;5~20 min,30%A;20→35 min,30%A→35%A;35→45 min,35%A→47%A;45→47 min,47%A→60%A;47→60 min,60%A);体积流速:1.0 mL·min^-1;柱温:23℃;蒸发光散射检测器(漂移管温度:90.0℃,气体流速:2.8 L·min^-1)。 结果: 黄姜素A、盾叶新苷、三角叶薯蓣皂苷、薯蓣皂苷和纤细皂苷5个皂苷均有良好的线性关系,平均加样回收率(n=6)分别为99.4%(RSD=2.8%),100.2%(RSD=2.0%),101.4%(RSD=1.2%),97.2%(RSD=2.1%),103.2%(RSD=2.4%)。 结论: 本方法简单、快速、结果可靠,可用于同时测定盾叶薯蓣根茎中上述5个成分的含量。

Objective: To establish an HPLC-ELSD method for simultaneous content determination of huangjiangsu A,zingiberensis new saponin,deltonin,dioscin,and gracillin to quantify the contents of the five saponins in the rhizome of Dioscorea zingiberensis C.H.Wright from different producing areas. Methods: The method was established using a Welchrom C₁₈ column(250 mm×4.6mm,5 μm),the mobile phase was acetonitrile(A)-water(B)with gradient elution(0-5 min,25%A→30%A;5-20 min,30%A;20-35 min,30%A→35%A;35-45 min,35%A→47%A;45-47 min,47%A→60%A;47-60 min,60%A),the flow rate was 1.0 mL·min^-1,the column temperature was 23℃,and the evaporative light scattering detector(ELSD)was selected as the detector(the temperature of drift tube was 90.0℃,and the gas flow rate was 2.8 L·min^-1). Results: The huangjiangsu A,zingiberensis new saponin,deltonin,dioscin,and gracillin had good linearities,and the average recoveries(n=6)were 99.4%(RSD=2.8%),100.2%(RSD=2.0%),101.4%(RSD=1.2%),97.2%(RSD=2.1%),103.2%(RSD=2.4%),respectively. Conclusion: This method is proved to be simple,fast and reliable,which can be used to determine the contents of the five saponins in the rhizome of D.zingiberensis.