HPLC法同时测定乌骨藤中通关藤苷A和D的含量

目的: 建立测定乌骨藤药材中通关藤苷A和D含量的HPLC方法,并对不同产地药材进行测定。 方法: 采用Agilent TC-C₁₈色谱柱(150 mm× 4.6 mm,5 μm),流动相为乙腈-0.05%磷酸(37∶63),流速1.0 mL·min^-1,检测波长210 nm,柱温25 ℃,进样量10 μL。 结果: 通关藤苷A和D的线性范围分别为3.0390~7.0910 μg (r=0.9997)和2.4651~5.7519 μg(r=0.9991),平均回收率(n=6)分别为 99.7%(RSD=2.6%)、99.4%(RSD=2.5%)。 结论: 本法准确,重复性好,专属性强,可控制药材的质量。且不同产地药材含有的2个成分的含量有一定差异性,其中云南含量最高,可指导临床用药。

Objective: To establish an HPLC method for the content determination of tenacissosides A and D in the stem of Marsdenia tenacissima (Roxb.)Wight et Arn.,and measure the herbs from different areas. Method: The analytical method was carried out on an Agilent TC-C₁₈ column (150 mm×4.6 mm,5 μm)with the mobile phase consisting of acetonitrile-0.05% phosphoric acid (37∶63)at a flow rate of 1.0mL·min^-1.The detection wavelength was 210 nm and column temperature was 25 ℃. Results: The calibration curves were linear within the range of 3.0390–7.0910 μg (r=0.9997)for tenacissoside A and 2.4651–5.7519 μg(r=0.9991)for tenacissoside D.The average recoveries (n=6)were 99.68%(RSD=2.57%)for tenacissoside A and 99.41%(RSD=2.46%)for tenacissoside D. Conclusion: The established method is accurate,reproducible and precise,which can be applied in the quality control of tenacissosides A and D in Marsdeniae Tenocissimae Caulis.The contents of two ingredients in herbs of different origins are different,which is the highest in samples from Yunnan;these results will benefit the clinical use of the drug.

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